Peanut somatic embryo induction and plant regeneration method

A peanut and plant technology, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve the problem of low frequency of plant regeneration, and achieve the effect of avoiding chimerism and high regeneration rate.
CN101965798AInactive Publication Date: 2011-02-09QINGDAO AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
QINGDAO AGRI UNIV
Publication Date
2011-02-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a peanut somatic embryo induction and plant regeneration method, which can solve the problem of low plant regeneration frequency in the prior art. The method of the invention comprises the following main steps: separating embryonic leaflet from a peanut embryo which is treated by surface sterilization and soaked, and inoculating the embryonic leaflet to an induction culture medium to which 2,4-D (5-10mg / L) is added for culturing a somatic embryo; then transferring the somatic embryo to a somatic embryo germinating culture medium to which BAP (4mg / L) is added, and subculturing twice to obtain regenerated plants; and cutting the somatic embryo at the bottom after the somatic embryo germinates and elongates, and transferring the cut somatic embryo to a 1 / 2MS inorganic salt and B5 organic rooting culture medium to which NAA (0.2-0.5mg / L) is added for inducing rooting to obtain complete plants. By taking the embryonic leaflet of peanut mature seeds as explant, and using the induction culture medium of 2,4-D (5-10mg / L) and the somatic embryo germinating culture medium of BAP (4mg / L), the somatic embryo induction rate and plant regeneration rate are high, and simultaneously the somatic embryo is derived from one cell, and the chimerism of regenerated plants is avoided.
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Description

technical field

[0001] The invention relates to a method for cultivating peanuts, in particular to a method for inducing somatic embryos of peanuts and regenerating plants. Background technique

[0002] Peanut is an important oil crop and economic crop in my country, and plays an important role in agriculture and even the entire national economy. However, due to the narrow genetic base and poor stress resistance of peanut cultivars, they are especially susceptible to various diseases and insect pests, which seriously affect their yield and quality. , The breeding of disease-resistant varieties has always been a difficult problem. At present, the genetic transformation and in vitro mutation breeding of resistance genes have received extensive attention at home and abroad. Whether gene transformation and in vitro mutagenesis can be successfully applied to peanut breeding depends critically on the establishment of an efficient plant regeneration system in peanut tissue cultur...

Claims

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