Artificial culture method of black cattle embryo

Abstract

The invention relates to an artificial culture method of a black cattle embryo, which comprises the following steps: taking a female black cattle ovary within 30min after slaughtering, and placing in a thermal insulation container containing sterile phosphate buffer solution at 38.5 DEG C, wherein 1UI / ml of antibiotics is contained in the container; using the sterile phosphate buffer solution at 38.5 DEG C to wash the ovary, removing redundant adipose tissues and cortex, using 75% alcohol to wash once, and using the sterile phosphate buffer solution to wash three times; further using a 5ml disposable syringe and a 18G needle head to extract an ovarian follicle with the diameter of 2-6mm, using an egg picking needle to pick up an oocyte under a stereomicroscope, and using mature culture fluid to wash the picked oocyte three times to five times; placing the oocyte in a mature culture medium, culturing under 5% CO2 and 38.5 DEG C saturation humidity conditions, and observing the maturation rate of the oocyte after 24h; and carrying out in vitro fertilization on the cultured oocyte and further forming the embryo. The method can fully utilize the oocyte of cattle after the slaughtering, and provide the convenience for artificial fertilization and culture of the black cattle embryo.

Description

Technical field [0001] The invention relates to an artificial breeding method of embryos, in particular to an artificial breeding method of black cattle embryos. Background technique [0002] Black cattle beef is a high-end beef with a broad market presence. Because cattle are mammals with a relatively long generation interval, the gestation period is as long as 285 days. For a long time, its slow reproduction speed has restricted the development of industrialized breeding Xuelong black cattle. And variety improvement. In order to solve the above problems, some adopt in vitro artificial insemination to cultivate embryos, and a large number of female black cattle oocytes are required during the operation. At present, in the artificial breeding of black cattle embryos, the oocytes of female black cattle after slaughter are not used, which is a waste. . Summary of the invention [0003] The purpose of the present invention is to provide a method for artificially cultivating black...

AI Technical Summary

Problems solved by technology

At present, in the artificial cultivation of black cattle embryos, the oocytes of slaughtered female black cattle are not used, which is a waste