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In situ hybridization detection kit for HER-2 gene, detection method and application thereof

A technology for HER-2 and detection kits, applied in the field of kits, can solve problems such as unreported detection technology, and achieve the effects of high sensitivity, convenient operation and strong specificity

Inactive Publication Date: 2011-03-30
SUZHOU FUYING GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report about the in situ hybridization detection kit and detection technology of HER-2 gene

Method used

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  • In situ hybridization detection kit for HER-2 gene, detection method and application thereof
  • In situ hybridization detection kit for HER-2 gene, detection method and application thereof
  • In situ hybridization detection kit for HER-2 gene, detection method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] An in situ hybridization detection kit for HER-2 gene, comprising a hybridization probe, a marker and a synergist, wherein the sequence of the hybridization probe is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0044] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0045] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0046] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0047] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0048] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0049] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0050] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0051] Chromogen A 175μl / tube 1 tube / box Yellow liquid

[0052] Chr...

Embodiment 2

[0094] A kind of HER-2 gene in situ hybridization detection method and its kit application

[0095] 1. Specimen processing

[0096] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0097] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0098] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0099] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with condit...

Embodiment 3

[0134] Parallel experiments between the detection of breast cancer disease with the HER-2 gene kit and the detection of breast cancer disease with the ERK gene kit.

[0135] In order to scientifically evaluate the specificity, sensitivity and accuracy of the above genes in breast cancer. We used the method of parallel experiments to detect the mRNA of the above genes at the same time. The detection technology used nucleic acid in situ hybridization technology, and used the breast tissue cells of the same patient with breast cancer to detect the mRNA of the HER-2 gene and the ERK gene at the same time (the nucleic acid source In situ hybridization, immunohistochemical staining, microscopic counting, result reporting, etc. all adopt the same methods, steps and reagents as the in situ hybridization techniques in Example 1 and Example 2). It is found that the expression level of HER-2 gene in patients with breast cancer is higher than that of ERK gene in patients with the same dis...

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Abstract

The invention relates to an in situ hybridization detection kit for a human epidermal growth factor receptor-2 (HER-2) gene. The kit comprises a hybridization probe and a marker, wherein the sequence of the hybridization probe is shown as SEQ ID NO.1. The invention also provides an in situ hybridization detection method for the Heparinase gene. In addition, the invention also provides the application of the kit in preparing medicaments for detecting diseases such as breast cancer. The in situ hybridization detection kit for the HER-2 gene and the detection method have the advantages that: the kit provided by the invention has the characteristics of high sensitivity and high specificity; and the detection method of the invention is convenient and easy to operate, and can be commonly used and popularized in district-level above hospitals.

Description

【Technical field】 [0001] The present invention relates to a kit, in particular to a HER-2 gene in situ hybridization detection kit and its detection method and application. 【Background technique】 [0002] According to the information provided by authoritative organizations at home and abroad, there are 1.6 million new cancer cases in my country every year, nearly 1.6 million deaths, and 6 million patients. Globally, there are 8 million new cancer patients every year, nearly 8 million deaths, and about 84 million patients. , by 2020 the number of people will double, which is a set of terrible figures. Breast cancer is a malignant tumor that seriously endangers women's health. In recent years, the incidence rate has steadily increased and tends to be younger. The incidence rate ranks third among female tumors. According to worldwide statistics, more than 1.2 million people around the world suffer from breast cancer every year, and breast cancer in industrialized countries has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张云福裘建英
Owner SUZHOU FUYING GENE TECH
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