Method for rapidly propagating miscanthus sacchariflorus through tissue culture

Abstract

The invention discloses a method for rapidly propagating miscanthus sacchariflorus through tissue culture, which comprises the steps of: A, selecting young ears: selecting young ears in a floret primordium forming stage; B, inducing with a primary inducing culture medium: cutting into small sections, disinfecting with 7 alcohol, then soaking with mercuric chloride, washing with sterile water, andinoculating the small sections on the primary inducing culture medium of the young ears for primary inducing to obtain embryogenic callus; C, subculturing with a seedling-growing culture medium: sub-seedling the obtained embryogenic callus on a subculture medium; D, inducing rooting with a rooting inducing culture medium: inducing the seedling for rooting on the rooting inducing culture medium; and E, acclimating and transplanting: in a greenhouse, periodically spraying acclimation culture liquid and clear water on the seedling every day, transplanting into a nursery garden, lighting, illuminating, and obtaining the miscanthus sacchariflorus. The method is simple and easy and convenient to operate, has the advantages of high differentiation callus rate and sprout rate as well as survival rate, and is suitable for laying the foundation of industrialized production and subsequent transgenosis of the miscanthus sacchariflorus.

Description

technical field [0001] The present invention relates to the technical field of tissue culture and rapid propagation of Miscanthus sacchariflorus ssp.lutarioriparius based on embryogenic callus, and more specifically relates to a method for tissue culture and rapid propagation of somatic embryos of Miscanthus sacchariflorus ssp. The implants obtain seedlings through callus induction, as well as multiplication, rooting, domestication and transplantation. Background technique [0002] In the 1990s, there were relevant reports on the tissue culture and rapid propagation technology of Miscanthus in China. They used young leaves, hypocotyls, young roots, and young ears as explants for the rapid propagation of Miscanths. , but their callus rate and differentiation rate are different, the reproduction efficiency of the young panicle is the highest, but no embryogenic callus is obtained. In addition, in 2005, there was also a report on the tissue culture and rapid propagation techno...

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Problems solved by technology

[0002] In the 1990s, there were relevant reports on the tissue culture and rapid propagation technology of Miscanthus in China. They used young leaves, hypocotyls, young roots, and young ears as explants for the rapid propagation of Miscanths. , but their callus rate and differentiation rate are different, the reproduction efficiency of the young panicle is the highest, but no embryogenic callus is obtained

In addition, in 2005, there was also a report on the tissue culture and rapid propagation technology of Miscanthus plants. The stem cutting technology was used to obtain seedlings, but this is also the organogenesis pathway used, and embryogenic callus was not obtained.

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