Method for producing newcastle disease, infectious bronchitis, egg drop syndrome and avian influenza (H9 subtype) combined inactivated vaccine

A technology for egg drop syndrome and bronchitis, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, virus antigen components, etc., can solve the problem of increasing the burden of manpower and material resources on chicken farms, increasing the susceptibility of chickens to diseases, Affect production performance and other issues, achieve the effect of long protection period, high potency, and reduce occurrence and spread

Inactive Publication Date: 2011-05-04
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are many commercial products on the market for the above four diseases, most of which are very simple traditional vaccines, which need to be used multiple times, which not only increase

Method used

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  • Method for producing newcastle disease, infectious bronchitis, egg drop syndrome and avian influenza (H9 subtype) combined inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] (1) Antigen preparation and inspection of semi-finished products

[0074] 1. Preparation of Chicken Newcastle Disease Virus Liquid

[0075] (1) Inoculation Take the virus seeds for production and make appropriate dilution with sterilized physiological saline (such as 10 -4 or 10 -5 ), inoculate 4997 10-11-day-old susceptible chicken embryos, 0.1ml per embryo, seal the pinhole after inoculation, and continue incubation at 36-37°C without turning the embryos.

[0076] (2) Incubation and observation After chicken embryos were inoculated, the embryos were photographed once a day, and the chicken embryos that died 60 hours ago were discarded. Thereafter, the embryos were photographed once every 4-6 hours, and the dead embryos were taken out at any time until 96 hours, regardless of whether they were dead or not, all were taken out, the air chamber was upward, and they were cooled at 2-8°C for 12-24 hours.

[0077] (3) Harvesting Take out the cooled chicken embryos, and ha...

Embodiment 2

[0123] Product testing

[0124] 1. traits

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PUM

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Abstract

The invention relates to a method for producing a newcastle disease, infectious bronchitis, egg drop syndrome and avian influenza (H9 subtype) combined inactivated vaccine. A newcastle disease virus La Sota strain, a gallinaceous infectious bronchitis virus M41 strain, an egg drop syndrome virus NE4 strain and an A-type avian influenza virus (H9 subtype) YBF003 strain serve as production virus strains, and are mixed with a high-quality adjuvant to be prepared into the inactivated vaccine through a super concentration process; after an animal is immunized by the vaccine, an antibody is rapidly produced, the titer is high, the protection period is long, and occurrence and spread of diseases are reduced.

Description

technical field [0001] The invention relates to a method for preparing an oil emulsion vaccine for preventing Newcastle disease, infectious bronchitis, egg drop syndrome and H9 subtype avian influenza in chickens, and belongs to the field of veterinary biological products. Background technique [0002] Newcastle disease (ND) has a high morbidity and fatality rate, and it occurs frequently all over the world. Once it breaks out, it will bring a devastating blow to the poultry industry. It is a major infectious disease that endangers the poultry industry. OIE lists it as a Category A disease. (Yin Zhen, Liu Jinghua, 1997. Animal Virology. Beijing. Science Press). [0003] Chicken infectious bronchitis (Infectious Bronchitis, IB) infection can lead to slow growth of chicks and broilers, decreased egg production and decreased egg quality of laying hens. The OIE lists it as a Category B disease. (Yin Zhen, Liu Jinghua, 1997. Animal Virology. Beijing. Science Press). [0004] ...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61P31/20A61K39/145A61K39/17A61K39/215A61K39/235
Inventor 李昌友宫晓李陆梅左青山徐宝娟
Owner YEBIO BIOENG OF QINGDAO
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