Method for identifying sampling points of biological chip on basis of edge detection

Abstract

The invention discloses a method for identifying sampling points of a biological chip on the basis of the edge detection. The method comprises the following steps of: firstly, determining a real area of the sampling points by edge extraction, edge connection and area filling; then meshing by adopting a scanning method and automatically obtaining the row number, the column number and the total number; and finally corresponding area positions of the sampling points in each determined mesh to an original drawing, carrying out automatic searching to obtain the area and the mean value of gray scale of the sampling points in the original drawing, and comparing the area and the value of gray scale of the sampling points in the original drawing with corresponding parameters of the sampling points in the standard chip to obtain an automatic identification result of the sampling points. The method has high positioning accuracy. Software of the method is simpler to implement. The method has short time of an algorithm, is convenient and rapid to operate and makes full automation and intellectualization of information extraction of the biological chip possible.

Description

technical field [0001] The invention belongs to the technical field of image recognition, and relates to a method for recognizing sample points of a biochip image, in particular to a method for recognizing sample points of a biochip image based on edge detection. Background technique [0002] A biochip is an orderly array of a series of addressable recognition molecules fixed at a certain position on the surface of a small substrate, and these spot molecules carry a large amount of biological information. Through screening and detection analysis, people can quickly obtain biological information in samples. Therefore, biochip technology has become an important tool for life scientists to study gene expression analysis, disease diagnosis, new drug discovery and many other fields. [0003] The biochip information processing process is to analyze the absorbance value, area or absorbance ratio of the sample points of the imaging results of the biochip. Important biological info...

AI Technical Summary

Problems solved by technology

[0004] In view of the defects that the existing technology needs to manually input the number of rows and columns of the chip array, the radius of the sample point and the row and column spacing of the array, the positioning of the sample point is not accurate, the data repeatability and reliability are difficult to be guaranteed, and the degree of automation is not high. The purpose of the present invention is to propose a kind of identification method based on edge detection biochip image samples

Images