Method for producing enramycin by fermentation
A technology of enramycin and fermentation broth, applied in the field of fermentation, can solve problems such as insufficient dissolved oxygen, and achieve the effect of improving fermentation level
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Embodiment 1
[0015] 1) Incline cultivation:
[0016] Streptomyces fungicidious CGMCC 4.1312 T (purchased from the Institute of Microbiology, Chinese Academy of Sciences) were inoculated on a solid slant medium under sterile conditions, and incubated at a constant temperature of 28°C for 8 days;
[0017] Wherein, the solid slant medium includes the following components: corn flour 20g / L, peptone 1g / L, ZnSO 4 .7H 2 O 0.1g / L, MnCl 2 .4H 2 O 0.02g / L, agar 15g / L.
[0018] 2) Fermentation culture:
[0019] Inoculate the bacteria cultured in step 1) on the fermentation medium in the fermentation bottle under aseptic conditions, ferment for 40 hours at 28° C., 300 r / min, and replenish water in the fermentation broth. The amount of water replenishment is 5% of the volume of the fermentation broth , the number of fermentation days is 8 days, and the average fermentation level is 850U / ml;
[0020] Wherein, the fermentation medium includes the following components: 100 g / L of corn flour, 10 g / L...
Embodiment 2
[0022] 1) Incline cultivation:
[0023] Streptomyces fungicidious CGMCC 4.1312 T ) was inoculated on a solid slant medium under sterile conditions, and incubated at a constant temperature of 28°C for 8 days;
[0024] Wherein, the solid slant medium includes the following components: corn flour 20g / L, peptone 1g / L, ZnSO 4 .7H 2 O 0.1g / L, MnCl 2 .4H 2 O 0.02g / L, agar 15g / L.
[0025] 2) Fermentation culture:
[0026] Inoculate the bacteria cultured in step 1) on the fermentation medium in the fermentation bottle under aseptic conditions, ferment for 72 hours at 28° C., 300 r / min, and replenish water in the fermentation broth. The amount of water replenishment is 10% of the volume of the fermentation broth , the number of fermentation days is 8 days, and the average fermentation level is 1210U / ml;
[0027] Wherein, the fermentation medium includes the following components: 100 g / L of corn flour, 10 g / L of corn steep liquor, 3 g / L of ammonium sulfate, 0.1 g / L of zinc sulfate...
Embodiment 3
[0029] 1) Incline cultivation:
[0030] Streptomyces fungicidious CGMCC 4.1312 T ) was inoculated on a solid slant medium under sterile conditions, and incubated at a constant temperature of 28°C for 8 days;
[0031] Wherein, the solid slant medium includes the following components: corn flour 20g / L, peptone 1g / L, ZnSO 4 .7H 2 O 0.1g / L, MnCl 2 .4H 2 O 0.02g / L, agar 15g / L.
[0032] 2) Fermentation culture:
[0033] Inoculate the bacteria cultured in step 1) on the fermentation medium in the fermentation bottle under aseptic conditions, ferment for 96 hours at 28° C., 300 r / min, and replenish water in the fermentation broth. The amount of water replenishment is 20% of the volume of the fermentation broth , the number of fermentation days is 8 days, and the average fermentation level is 960U / ml;
[0034] Wherein, the fermentation medium includes the following components: 100 g / L of corn flour, 10 g / L of corn steep liquor, 3 g / L of ammonium sulfate, 0.1 g / L of zinc sulfate,...
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