Fermentation technology of anti-senile dementia pharmaceutical ingredient huperzine

A technology for huperzine A and senile dementia, applied in the direction of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of low yield of finished products, difficult industrial production, etc., and achieve the effect of increasing fermentation yield

Inactive Publication Date: 2011-09-21
HUNAN BAILUN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, some institutions and individuals at home and abroad are carrying out technical research on the production of huperzine A by fermenting various endophytes (including fungi and bacteria) of Huperzia plants, but the yield of finished products in experimental research has been very low, making it It is difficult to enter industrial production, but our research on this technical project has obtained the optimal formula and innovative process. By adding the precursor substance 2-methylene-1,3-propanediol diacetate, the fermentation of huperzine A Yield increased by 12.5%-18.7%, which is of great significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the fermentation technology of anti-senile dementia crude drug huperzine A: (concrete grams or other specific numerical values ​​will be used in the embodiment)

[0029] 1. Inoculate the huperzine A-producing bacteria stored at low temperature on fresh PDA slant medium, and culture at 28°C for 4 days.

[0030] 2. Pick and inoculate the cultured mature slant strains into the liquid shake flask seed medium, and incubate at 28°C and 150r / min at constant temperature for 4 days.

[0031] 3. Inoculate the cultured shake flask liquid seed culture medium into the workshop seed tank culture medium, cultivate for 3 days at 28° C., with an aeration rate of 1:1 VVM.

[0032] 4. Accurately weigh various raw materials according to the formula of the fermentation medium, and prepare the fermentation medium. After sterilizing and cooling, insert the cultured liquid seeds at 30°C, the stirring speed is 200r / min, and the amount of seeds inserted is 10%. The pH value was co...

Embodiment 2

[0052] Embodiment 2: the fermentation technology of anti-senile dementia crude drug huperzine A: (concrete grams or other specific numerical values ​​will be used in the embodiment)

[0053]1. Inoculate the huperzine A-producing bacteria stored at low temperature on fresh PDA slant medium, and culture at 28°C for 4 days.

[0054] 2. Pick and inoculate the cultured mature slant strains into the liquid shake flask seed medium, and cultivate them with constant temperature shaking at 28°C and 180r / min for 3.5 days.

[0055] 3. Inoculate the cultured shake flask liquid seed culture medium into the workshop seed tank culture medium, cultivate for 4 days at 28° C., with an aeration rate of 1:1.1 VVM.

[0056] 4. Accurately weigh various raw materials according to the formula of the fermentation medium, and prepare the fermentation medium. After sterilizing and cooling, insert the cultured liquid seeds at 30°C, the stirring speed is 230r / min, and the amount of seeds inserted is 10%. ...

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Abstract

The invention discloses a fermentation technology of anti-senile dementia pharmaceutical ingredient huperzine. Acremonium rndophytium of which the preservation number is CCTCC M206118 serves as a production strain. In the fermentation process, the fermentation yield of the huperzine can be improved by 12.5 to 18.7 percent by adding a precursor, namely 2-methylene-1,3-propylene glycol diacetate. The formula of a seed medium comprises the following components in percentage by weight: 2 percent of corn flour, 2 percent of glucose, 0.8 percent of corn steep liquor, 1 percent of yeast powder, 1.65 percent of bean cake powder, 0.2 percent of KH2PO4, 0.05 percent of MgSO4.7H2O and 0.35 percent of CaCO3. The formula of a fermentation medium comprises the following components in percentage by weight: 4 percent of corn flour, 2 percent of maltose, 2 percent of corn starch, 1.2 percent of yeast powder, 0.6 percent of corn steep liquor, 1.55 percent of bean cake powder, 0.1 percent of KH2PO4, 0.06 percent of MgSO4.7H2O, 0.05 percent of NaCl and 0.4 percent of CaCO3.

Description

technical field [0001] The invention relates to a fermentation technology of huperzine A, an anti-senile dementia raw material medicine. Background technique [0002] Patent technology published on June 11, 2008: Huperzia serrata endophyte and its application (patent application number 200610119149.7; publication number CN101195804A) discloses an endophyte that can produce huperzine A——Acremonium acremonium (Acremonium endophytium), the deposit number is CCTCC M 206118. It also relates to the technical application of producing huperzine A by fermentation of the bacteria. [0003] At present, some institutions and individuals at home and abroad are carrying out technical research on the production of huperzine A by fermenting various endophytes (including fungi and bacteria) of Huperzia plants, but the yield of finished products in experimental research has been very low, making it It is difficult to enter industrial production, but our research on this technical project ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/12C12R1/645
Inventor 兰时乐肖宏王玲
Owner HUNAN BAILUN TECH
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