Protein preparation method

A protein and group technology, applied in the field of peptide connection, can solve the problems of high corrosiveness, inability to synthesize efficiently, and the method of protein synthesis needs to be improved.
CN102199214BActive Publication Date: 2013-05-22TSINGHUA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2013-05-22

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Abstract

A protein preparation method provided by the invention comprises the following steps to connect a first peptide with hydrazide group on C terminus and a second peptide with cysteine on N terminus: mixing the first peptide and the second peptide in a solution containing NaNO2 and adjusting the mixed solution pH to an acidic pH so as to converting the hydrazide group of the first peptide into acyl azide group to obtain a first reaction mixture; adding a solution containing a reducing agent into the first reaction mixture and adjusting the pH to a neutral pH, wherein the reducing agent contains mercapto group, so as to convert the acyl azide group of the first peptide into thioester group, and maintaining the neutral pH so as to conducting a connection reaction between the thioester on the Cterminus of the first peptide and the N terminus of the second peptide to produce proteins. A method for preparing polypeptide thioester compounds is also provided. According to the protein preparation method provided by the invention, proteins can be effectively synthesized.
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Description

technical field

[0001] The present invention relates to the preparation of proteins and methods of proteins, more particularly to methods of linking peptides. Background technique

[0002] The relationship between protein structure and function is one of the frontier hot issues in biology, and the acquisition of high-purity or specific non-naturally modified proteins can greatly promote the development of this field. Chemical synthesis and semi-synthesis of proteins are considered to be important ways to obtain target proteins with specific structural modifications, so the development of efficient synthesis techniques is of great academic and commercial value. As early as 1901, Fisher, a master of organic synthesis, proposed the idea of ​​chemically synthesizing biological enzymes. In 1963, Merrifield developed solid-phase synthetic peptide technology to realize the chemical synthesis of biologically active peptides. However, due to the serious aggregation of long-chain po...

Claims

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