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Bacillus subtilis submerged fermentation culture medium applied to microbial fertilizers

A technology of Bacillus subtilis and microbial fertilizer, which is applied in the field of microbial cultivation, can solve the problems of high raw material cost and complicated medium preparation, and achieve the effects of simplifying operation, reducing production cost and improving product quality

Active Publication Date: 2013-01-16
MAANSHAN KEBANG ECO FERTILIZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of this fermentation medium is that the preparation of the medium is relatively complicated and the cost of raw materials is high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The following ratio (by weight) of medium is used: 8 kg of wheat flour, 16 kg of bran, and the volume is adjusted to 0.8M3 with water. After stirring evenly, sterilize at 121°C for 25 minutes, cool to 25-40°C, add Bacillus subtilis seed liquid at 5% (weight ratio), fermentation conditions: 37±2°C, ventilation ratio 1:1.0 volume / volume / 200-250 rpm, 40 hours of fermentation, residual sugar (determined by anthrone-sulfuric acid method) is 5% of that before inoculation, bacterial concentration (determined by plate dilution method, three times in parallel) is an average of 3.85 billion CFU / ml, The sporulation rate is about 90%.

Embodiment 2

[0018] Use the following ratio (by weight) medium: 12 kg of wheat flour, 14 kg of bran, dilute to 0.8M with water 3 . After stirring evenly, sterilize at 121°C for 25 minutes, cool to 25-40°C, add Bacillus subtilis seed liquid at 5% (weight ratio), fermentation conditions: 37±2°C, ventilation ratio 1:1.0 volume / volume / 280-320 rpm, 48 hours of fermentation, residual sugar (determined by anthrone-sulfuric acid method) was 8% of that before inoculation, bacterial concentration (determined by plate dilution method, three times in parallel) averaged 4.24 billion CFU / ml, The sporulation rate is about 95%.

Embodiment 3

[0020] The following ratio (by weight) of the culture medium is used: 7.2 kg of wheat flour, 17 kg of bran, and the volume is adjusted to 0.8M3 with water. After stirring evenly, sterilize at 121°C for 25 minutes, cool to 25-40°C, add Bacillus subtilis seed liquid at 5% (weight ratio), fermentation conditions: 37±2°C, ventilation ratio 1:1.0 volume / volume / 300-350 rpm, 38 hours of fermentation, residual sugar (determined by anthrone-sulfuric acid method) is 3% of that before inoculation, bacterial concentration (determined by plate dilution method, three times in parallel) is an average of 4.56 billion CFU / ml, The sporulation rate is about 90%.

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Abstract

The invention relates to a bacillus subtilis submerged fermentation culture medium for compound microbial fertilizers. The culture medium comprises the following components in percentage by weight: 0.8 to 1.5 percent of wheat powder, and 1.5 to 4 percent of bran powder. The composition of the culture medium is simple, the pH value is not required to be adjusted during the preparation, and the culture medium can promote the growth of thalli and the formation of spores, so that the quality of products is improved and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and relates to a process technology for forming spores of bacillus subtilis industrialized liquid, in particular to a submerged fermentation medium of bacillus subtilis applied to microbial fertilizers. Background technique [0002] Bacillus subtilis plays an important role in promoting crop growth and enhancing crop disease resistance, and is a safe microorganism, so it is widely used in the production of microbial fertilizers. [0003] Because spores have the advantage of strong stress resistance, most of the microorganisms contained in microbial fertilizers exist in the form of spores. It is easy to produce Bacillus subtilis spores by solid fermentation, but the labor intensity is high, and the concentration of spores is not high, generally 0.1-100 million CFU / g. The People's Republic of China Agricultural Industry Standard NY / T 798-2004 (Compound Microbial Fertilizer) stipu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 杨柳唐旺全杨红兵
Owner MAANSHAN KEBANG ECO FERTILIZER
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