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Method for creating new peanut specie in space breeding

A technology of aerospace breeding and new germplasm, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of low mutagenesis beneficial mutation rate, difficult selection, high cost of carrying, etc. The method is simple and easy to master , low loading cost, low selection difficulty

Inactive Publication Date: 2011-11-23
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem to be solved by the present invention is to provide a new material for peanut space breeding, so as to overcome the problems of low mutagenesis beneficial mutation rate, high carrying cost and difficult selection under the existing conditions

Method used

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Examples

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Effect test

Embodiment 1

[0013] A method for aerospace breeding to create new peanut germplasm. After selecting Huayu No. 20 peanuts mature, select 100 full seeds, first disinfect them with 70% alcohol for 1 minute, and then use 0.1% by mass Sterilize with HgCl for 6 minutes, perform surface disinfection, rinse with sterile water 4 times, remove the seed coat, remove two cotyledons, and obtain the peanut embryo cut off the front embryo growth point as a material for space breeding. The embryo growth point after mutagenesis on the return spacecraft was inoculated on MSB5 medium supplemented with 1mg / L NAA and 4mg / L 6-BA to induce callus, and transferred to the supplemented with 1mg / L 6-BA, 0.5mg after 20 days. Regenerated shoots were obtained on the MSB5 bud differentiation medium containing 2 mg / L KIN and 2 mg / L NAA. The tissue culture regenerated seedling after the embryo growth point mutagenesis is a scion, the height of the seedling is 2-2.5 cm, the regenerated seedling is cut from the base of the b...

Embodiment 2

[0015] A method for aerospace breeding to create new peanut germplasm. After selecting Huayu No. 20 peanuts mature, select 100 full seeds, first disinfect them with 70% alcohol for 1 minute, and then use 0.1% by mass Sterilize with HgCl for 6 minutes, perform surface disinfection, rinse with sterile water 4 times, remove the seed coat, remove two cotyledons, and obtain the peanut embryo cut off the front embryo growth point as a material for space breeding. The embryo growth point after mutagenesis on the return spacecraft was inoculated on MSB5 medium supplemented with 1mg / L NAA and 4mg / L 6-BA to induce callus, and transferred to the supplemented with 1mg / L 6-BA, 0.5mg after 20 days. Regenerated shoots were obtained on the MSB5 bud differentiation medium containing 2 mg / L KIN and 2 mg / L NAA. The tissue culture regenerated seedling after the embryo growth point mutation is a scion, the seedling height is 2.5 cm, the regenerated seedling is cut from the base of the bud cluster, ...

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Abstract

The invention belongs to the technical field of the peanut genetic breeding method and relates to a method for creating a new peanut specie in space breeding. The method is characterized by comprising the following steps: the growing point of the mature peanut seed embryo is used as the carrying material to be carried in a returnable spacecraft for space mutation; and after the mutation, the mutated embryo growing point is used to perform tissue culture and obtain a tissue culture regeneration seedling, the tissue culture regeneration seedling is used as the scion, an unmutated seedling of the same variety is used as the rootstock to perform grafting treatment and form a grafted seedling, the grafted seedling is transplanted in a field and a new and beneficial mutated specie is obtained after harvesting. The method has the following beneficial effects: the mutagenic beneficial mutation rate is high, the carrying cost is low, the selection difficulty is small, and the method is simple and is easy to master.

Description

Technical field [0001] The invention belongs to the technical field of peanut genetic breeding methods, and relates to a method for aerospace breeding to create new peanut germplasm. Background technique [0002] Peanut is an important oil crop and economic crop in my country, and it is also the only bulk oil crop with competitiveness in the international market. China is the world's largest producer, consumer and exporter of peanuts. my country has a high level of peanut production, good planting efficiency, and great development potential. It plays an obvious role in ensuring the safe and effective supply of national edible vegetable oil. However, traditional breeding methods can hardly improve the yield and quality of peanuts. New breeding methods must be used to increase the yield and quality of peanuts in my country. The development of aerospace breeding technology provides an opportunity for flower breeding. [0003] Space breeding is also called space mutation breeding. It...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/06A01G1/00
Inventor 杨庆利禹山林潘丽娟朱凤杨珍陈明娜
Owner SHANDONG PEANUT RES INST
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