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Method for creating new peanut specie in space breeding

A space breeding and new germplasm technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low beneficial mutation rate of mutagenesis, difficult selection, high cost of carrying, etc. The method is simple and easy to master , low loading cost, low selection difficulty

Inactive Publication Date: 2012-11-14
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem to be solved by the present invention is to provide a new material for peanut space breeding, so as to overcome the problems of low mutagenesis beneficial mutation rate, high carrying cost and difficult selection under the existing conditions

Method used

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Examples

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Effect test

Embodiment 1

[0013] A method for space breeding to create new germplasm of peanuts. After the Huayu No. 20 peanuts are matured, 100 plump seeds are selected, firstly disinfected with 70% alcohol for 1 minute, and then 0.1% by mass. Sterilize with HgCl for 6 minutes, carry out surface disinfection, rinse with sterile water 4 times, take off the seed coat, and remove two cotyledons to obtain peanut embryos and cut off the front embryo growth point as the material for space breeding. Embryo growth points after mutagenesis carried by returnable spacecraft were inoculated on MSB5 medium supplemented with 1mg / L NAA and 4mg / L 6-BA to induce callus, and transferred to the medium supplemented with 1mg / L 6-BA and 0.5mg / L 6-BA 20 days later. / L KIN and 2mg / L NAA MSB5 shoot differentiation medium to obtain regenerated shoots. The tissue-cultured regenerated seedlings after embryonic growth point mutagenesis are scions with a seedling height of 2-2.5 cm. The regenerated seedlings are cut from the base ...

Embodiment 2

[0015] A method for space breeding to create new germplasm of peanuts. After the Huayu No. 20 peanuts are matured, 100 plump seeds are selected, firstly disinfected with 70% alcohol for 1 minute, and then 0.1% by mass. Sterilize with HgCl for 6 minutes, carry out surface disinfection, rinse with sterile water 4 times, take off the seed coat, and remove two cotyledons to obtain peanut embryos and cut off the front embryo growth point as the material for space breeding. Embryo growth points after mutagenesis carried by returnable spacecraft were inoculated on MSB5 medium supplemented with 1mg / L NAA and 4mg / L 6-BA to induce callus, and transferred to the medium supplemented with 1mg / L 6-BA and 0.5mg / L 6-BA 20 days later. / L KIN and 2mg / L NAA MSB5 shoot differentiation medium to obtain regenerated shoots. The tissue-cultured regenerated seedlings after embryo growth point mutagenesis were scions with a seedling height of 2.5 cm. The regenerated seedlings were cut from the base of ...

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Abstract

The invention belongs to the technical field of the peanut genetic breeding method and relates to a method for creating a new peanut specie in space breeding. The method is characterized by comprising the following steps: the growing point of the mature peanut seed embryo is used as the carrying material to be carried in a returnable spacecraft for space mutation; and after the mutation, the mutated embryo growing point is used to perform tissue culture and obtain a tissue culture regeneration seedling, the tissue culture regeneration seedling is used as the scion, an unmutated seedling of the same variety is used as the rootstock to perform grafting treatment and form a grafted seedling, the grafted seedling is transplanted in a field and a new and beneficial mutated specie is obtained after harvesting. The method has the following beneficial effects: the mutagenic beneficial mutation rate is high, the carrying cost is low, the selection difficulty is small, and the method is simple and is easy to master.

Description

technical field [0001] The invention belongs to the technical field of peanut genetic breeding methods, and relates to a method for creating new peanut germplasm through aerospace breeding. Background technique [0002] Peanut is an important oil crop and economic crop in my country, and it is also the only bulk oil crop with competitiveness in the international market. China is the world's largest peanut producer, consumer and exporter. my country's peanut production level is high, the planting benefits are good, and the development potential is great. It plays a significant role in ensuring the safe and effective supply of edible vegetable oil in the country. However, traditional breeding methods are difficult to qualitatively improve the yield and quality of peanuts, and new breeding methods must be used to increase the yield and improve the quality of peanuts in my country. The development of space breeding technology provides an opportunity for peanut breeding. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G1/06A01G1/00
Inventor 杨庆利禹山林潘丽娟朱凤杨珍陈明娜
Owner SHANDONG PEANUT RES INST
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