A complementary color photometric automatic titration device for reducing sugar concentration determination

An automatic titration and sugar concentration technology, which is applied in measuring devices, color/spectral characteristic measurement, and material analysis through optical means, can solve the problems of inconspicuous color change, inability to judge the end point, large human error, etc., and achieve simple operation , Reagent measurement is accurate and fast, and the effect of small error

Inactive Publication Date: 2011-11-30
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are cumbersome and time-consuming, and sometimes there are large human errors, especially for turbid samples or samples with darker color, the color change is not obvious, and the end point cannot be judged

Method used

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  • A complementary color photometric automatic titration device for reducing sugar concentration determination
  • A complementary color photometric automatic titration device for reducing sugar concentration determination
  • A complementary color photometric automatic titration device for reducing sugar concentration determination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0044] The main performance and sample test of the complementary color photometric automatic titration device

[0045] 1. Precision and accuracy

[0046] (1) Materials and methods

[0047] Complementary color reducing sugar automatic titration device

[0048] Power on: Press the "ON / OFF" button to start the preparation procedure automatically.

[0049] Calibration: Press the Calibration key to automatically enter the calibration program. After the reagent pump is completed, use a micro-syringe to inject the standard into the reaction pool, and the instrument will automatically calibrate and print after completion;

[0050] Determination: Press the determination key to automatically enter the determination program. After the reagent pump is completed, use a micro syringe to inject the standard into the reaction pool. After completion, the instrument automatically calibrates and prints;

[0051] (2) Results and analysis

[0052] With 1.0% glucose standard solution, the inj...

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PUM

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Abstract

The invention relates to a color-complementing photometric titration device capable of quickly and accurately measuring the concentration of reducing sugar in various samples, and belongs to the technical field of biochemical analysis instruments. The reducing sugar in the sample and Fehling’s reagent or reducing sugar and potassium ferricyanide-sodium hydroxide react in a slight boiling state. Reducing sugar will change the color of the reaction system. The reaction solution system is irradiated with a light source system with a complementary color to the reaction solution system, and a photoelectric conversion device is used to detect the transmitted light. At the end of the reaction, the received signal will change suddenly. The complementary color photometric titration established by the above method The device can be used for rapid determination of reducing sugar concentration in various samples. Complementary color photometry reduces light transmittance fluctuations, increases the intensity of mutation signals, and has almost no interference signals. Therefore, the method for determining reducing sugar concentration is not affected by sample color, turbidity, and heating bubbles.

Description

technical field [0001] The invention relates to a color-complementing photometric titration device capable of quickly and accurately measuring the concentration of reducing sugar in various samples, and belongs to the technical field of biochemical analysis instruments. Background technique [0002] Reducing sugars refer to reducing sugars that contain free aldehyde or ketone groups in the molecule. Among sugars, monosaccharides (glucose, fructose) and lactose and maltose in disaccharides all have reducing groups (aldehyde groups or ketone groups), so they are reducing sugars; as long as disaccharides, trisaccharides and even polysaccharides have reducing ends, Also reducing sugar. Therefore, reducing sugar refers to a general term for a class of reducing sugars containing free aldehyde or ketone groups in the molecule. Many sugars have no reducing ends, such as sucrose in disaccharides, dextrin in polysaccharides, starch, cellulose, etc., which are not reducing in themsel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/27G01N21/01
Inventor 马耀宏孟庆军杨俊慧杨艳张利群史建国
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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