A method and kit for detecting amylase

A detection method and amylase technology, which are applied in the field of medical testing and determination, can solve the problems such as the reduction of ingredients and the reduction of the accuracy of detection results, and achieve the effects of long shelf life, easy popularization and use, and good stability.

Active Publication Date: 2011-12-21
CHANGCHUN HUILI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the chromogenic agent iodine solution in the above method is easy to sublimate and volatilize, causing the components to...

Method used

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  • A method and kit for detecting amylase
  • A method and kit for detecting amylase
  • A method and kit for detecting amylase

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Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: A kit for detecting amylase according to the present invention.

[0023] The kit for detecting amylase in the present invention is multi-reagent.

[0024] The first reagent is a dry powder reagent, including 960mg / L soluble starch, 50mmol / L pH7.2 phosphate buffer and 60mmol / L sodium benzoate;

[0025] The second reagent is 30mmol / L potassium iodide;

[0026] The third reagent is 8 mmol / L potassium iodate solution at pH 2.0.

Embodiment 2

[0027] Embodiment 2: A kit for detecting amylase according to the present invention.

[0028] The kit for detecting amylase in the present invention is multi-reagent.

[0029] The first reagent is a dry powder reagent, including 960mg / L soluble starch, 20mmol / L pH7.2 phosphate buffer and 50mmol / L sodium benzoate;

[0030] The second reagent is 50mmol / L potassium iodide;

[0031] The third reagent is 12mmol / L potassium iodate solution at pH3.0.

Embodiment 3

[0032] Embodiment 3: A kit for detecting amylase according to the present invention.

[0033] The kit for detecting amylase of the present invention is a double reagent.

[0034] The first reagent is a dry powder reagent, including 960mg / L soluble starch, 100mmol / L pH7.2 phosphate buffer, 48mmol / L potassium iodide and 70mmol / L sodium benzoate;

[0035] The second reagent is 8 mmol / L potassium iodate solution at pH 2.5.

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Abstract

The invention relates to the technical field of medical detection and discloses a method and a kit for detecting amylase. The method for detecting the amylase provided by the invention comprises the following steps of: in a buffer solution with a pH value of 7.2, mixing a sample to be detected and soluble starch to obtain a mixed solution; mixing potassium iodide and acidic potassium iodate and mixing the mixture of the potassium iodide and the acidic potassium iodate with the obtained mixed solution; detecting absorbance in a 630nm wavelength; and calculating the concentration of the amylase in the sample to be detected according to the absorbance of a non-enzymatic blank tube which is subjected to the same treatment. In the method, the potassium iodide and the potassium iodate generate iodine under the acidic condition, and the iodine is combined with the unreacted starch to form a blue compound, so influence on reaction color development due to reduction of components caused by easiness in sublimation and volatilization of an iodine solution which is directly used is avoided, and the accuracy of a detection result is improved. The kit is good in stability, long in preservation time, wide in application range and convenient for popularization and can be applied to determination of the content of the amylase in serum or urine by hospitals, health departments and medical biological scientific research institutes at all levels.

Description

technical field [0001] The invention relates to the technical field of medical testing and determination, in particular to a method and kit for detecting amylase. Background technique [0002] Amylase (α-Amylase), referred to as AMS or AMY, is a type of enzyme that can hydrolyze starch molecules. It generally acts on α-1,4-glucans such as soluble starch, amylose, and glycogen to hydrolyze α-1, 4-Glycosidic bonds, resulting in dextran, maltose and glucose molecules. Amylase is abundant in the pancreas and salivary glands. Pancreatic amylase is excreted into the digestive tract in an active state by the pancreas. It is the most important enzyme that hydrolyzes carbohydrates. It belongs to α-amylase like the amylase secreted by salivary glands. Amylase has a small molecular weight and can be filtered through the glomerulus and excreted with urine. When suffering from pancreatic diseases or pancreatic exocrine dysfunction, amylase activity can be increased or decreased. Theref...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 董理
Owner CHANGCHUN HUILI BIOTECH CO LTD
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