Solid-phase pouring enrichment method and supporting equipment

Abstract

The solid-phase pouring enrichment method is to replace the liquid enrichment solution with solid-phase enrichment medium and swabs containing solid-phase enrichment medium, and to cultivate and isolate pathogenic bacteria from the original sample containing miscellaneous bacteria by pouring culture method . The supporting device is a container containing solid-phase enrichment medium and a swab containing medium, so that it has the functions of sampling, pre-enrichment, enrichment, and biochemical rough screening at the same time. Take the Salmonella detection tube used in the physical examination of practitioners as an example, moisten the anal swab containing the culture medium with water, directly sample and culture, and directly observe the reaction. If Salmonella is suspected, there will be colored suspicious spots on the swab. Take the spots for pure isolation, and then do further identification. Those without spots are negative, and there is no need to transfer to the plate. The detection rate of this method is significantly higher than that of the conventional method, and because the observation time can be extended, the delayed growth bacteria will not be missed. This method simplifies procedures, reduces costs, and has a high detection rate. Another Salmonella detection bag according to the same principle has a higher detection rate. A series of multi-functional products can also be developed, such as bacteria monitoring in food, food poisoning detection, infectious disease detection, nosocomial infection monitoring and other products.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms in a laboratory and a detection device, in particular to a solid-phase pouring bacterium enrichment method and a supporting device. Background technique [0002] At present, when detecting bacteria, swabs are often used for sampling, and then liquid enrichment solution is used for enrichment. The swab used for sampling is only used as a delivery tool, and the sample is then transferred to normal saline or enrichment solution. For example, for Salmonella screening during the physical examination of practitioners, the traditional method is to take samples from anal swabs and place them in the enrichment solution to enrich the bacteria, then transfer them to flat plates for pure isolation, and then select suspicious colonies for further identification. This traditional method is small but the procedures are complicated, and there are many problems to be improved in the liquid enrichment sol...

AI Technical Summary

Problems solved by technology

This traditional method is small but the procedures are complicated, and there are many problems to be improved in the liquid enrichment solution, such as supporting the injured bacteria, neutralizing the antibiotics in the sample, and delaying the observation of the delayed growth bacteria. The problem is that only a very small part of the samples from the previous step can be used in the next step, which has high randomness and poor representativeness, so the detection rate is extremely low, and no matter whether there is suspicious Salmonella in the enrichment solution, each sample must be transferred to a plate for purification. separate, waste

In order to solve the existing problems, although a utility model patent (Patent No. ZL 2007200879654) has been applied for, it is only the improvement of the shape and structure, which cannot fundamentally solve the above-mentioned existing problems

Images