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Method for extracting iridoid active ingredients from plantain seeds

A technology of iridoids and psyllium extract, which is applied in the field of natural medicine, can solve the problems of complex components of psyllium and difficulties in extraction and separation, and achieve good application prospects

Inactive Publication Date: 2012-02-15
BEIJING COLLAB PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the components of psyllium are complex, and many compounds have similar structures and properties. Therefore, it is difficult to extract and separate iridoid active ingredients from psyllium.
There is no report on the method of extracting iridoids from Semen Plantaginis at present

Method used

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  • Method for extracting iridoid active ingredients from plantain seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Extraction: Fructus Plantaginis pulverizes, crosses 20 mesh sieves, takes by weighing 1000g and adopts ultrasonic extraction, adds 70% ethanol with 10 times of liquid-solid ratio, ultrasonic (frequency 80KHz) extracts 30min, repeats extraction 3 times, suction filtration, The extracts were combined, the ethanol was recovered and concentrated to 1000ml, cooled to room temperature, allowed to stand for 24 hours, centrifuged and filtered to obtain a clear filtrate.

[0026] (2) Refining: the above-mentioned filtrate is added to the pretreated HP-20 type macroporous resin column with a flow rate of 0.5BV / h, washed with water, 5%, 30% and 75% concentration of ethanol in sequence, HPLC For detection, collect the eluate rich in iridoids (washed with water and elute with 5% ethanol), combine, concentrate to a thick paste, and dry in vacuum to obtain the total iridoid extract of Semen Plantaginis. The content of the iridoids is 52% as measured by ultraviolet (UV) method.

Embodiment 2

[0028] (1) Extraction: Fructus Plantago is pulverized, crosses 20 mesh sieves, weighs 1000g and extracts with 70% ethanol heating under reflux, adds 70% ethanol (containing 0.5% HCl) with 4 times of liquid-solid ratio each time, and refluxes for the first time 2h, the second and third reflux for 1h. Suction filtration, combined extracts, recovered ethanol and concentrated to 1000ml, cooled to room temperature, let stand for 24h, centrifuged and filtered to obtain clarified filtrate.

[0029] (2) Refining: the above-mentioned filtrate is added in the pretreated HP-20 type macroporous resin column with the flow velocity of 1BV / h, and the ethanol of water, 5%, 30% and 75% concentration is successively gradient washed, and HPLC detects, Collect the water-washed and 5% ethanol parts rich in iridoids, combine and concentrate to a certain volume, extract with n-butanol, recover n-butanol, concentrate to extract, and vacuum-dry to obtain total iridoids of plantago class extract. The...

Embodiment 3

[0031] (1) Extraction: Fructus Plantaginis is softened with steam, dried, pulverized, sieved, weighed Fructus Plantaginis powder 1000g after burying, adopts decoction to extract, adds deionized water with 40 times of liquid-solid ratio, Extract for 2 hours each time, repeat the treatment 3 times, filter and combine the extracts, recover ethanol and concentrate to 1000ml, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.

[0032] (2) Refining: the above-mentioned filtrate is added to the pretreated polyamide column at a flow rate of 2BV / h, and then the mother liquor and washing liquid flowing out from the polyamide column are added to the pretreated polyamide column at a flow rate of 1BV / h. HP-20 type macroporous adsorption resin, washed with water, ethanol of 5%, 30% and 75% concentration gradient successively, HPLC detects, collects the water washing and 5% ethanol elution part rich in iridoids, merges, reduces Concentrate...

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Abstract

The invention relates to the technical field of natural medicaments, and in particular relates to a method for extracting iridoid active ingredients from plantain seeds. In the method provided by the invention, a plantain seed extract is obtained through adopting a solvent for extraction and refining with a chromatographic process or a combination of chromatography and a solvent extraction method. The prepared plantain seed extract contains more than 50% by weight of iridoid active ingredients. The method is simple and has an industrial application prospect.

Description

technical field [0001] The invention relates to the technical field of natural medicines. Specifically, it relates to a method for extracting iridoid active ingredients from psyllium. Background technique [0002] Plantago is one of the traditional medicinal materials in my country. It first appeared in "The Book of Songs", and its medicinal use was first recorded in "Shen Nong's Herbal Classic" and was listed as the top grade of medicine. In addition, there are also called plantain, oxtonus, wheel dish, pig's ear grass and so on. Semen Plantago is Plantago depressa Willd. (Plantago depressa Willd.), also known as Plantago asiatica Linn. (Plantago asiatica Linn.), also known as the dried seeds of Plantago, which is distributed all over the country and has extensive resources . It has the effects of diuresis and stranguria, dampness and diarrhea, liver clearing and eyesight clearing, heat clearing and phlegm resolving. Semen psyllium contains a large amount of mucus (poly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 王珂赵大龙陈明霞王振辉
Owner BEIJING COLLAB PHARMA
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