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Construction method of drug evaluation model for dermal pathology of tuberculosis rabbit

A technology for evaluating models and building methods, applied in the medical field, to achieve the effect of stable model and obvious symptoms

Inactive Publication Date: 2012-05-02
LANZHOU UNIVERSITY
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  • Description
  • Claims
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Problems solved by technology

Effective and intuitive animal models are currently lacking to evaluate drugs against tuberculosis immunopathology

Method used

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  • Construction method of drug evaluation model for dermal pathology of tuberculosis rabbit
  • Construction method of drug evaluation model for dermal pathology of tuberculosis rabbit
  • Construction method of drug evaluation model for dermal pathology of tuberculosis rabbit

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Embodiment Construction

[0025] Preferred embodiments of the present invention are described below, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0026] 1. Experimental materials and methods

[0027] 1. Experimental materials

[0028] (1) Experimental strain: Bacillus Calmette-Guerin (BCG), made into 5×10 before use 7 CFU / ml (the total number of bacterial communities per milliliter sample) is ready for use.

[0029] (2) Drugs: recombinant human interleukin-2 (IL-2), recombinant human interferon-r (IFN-r), cyclophosphamide, dexamethasone sodium phosphate injection. The medicine is subpackaged as follows, and dissolved with normal saline before each use.

[0030] The drug specifications and packaging dosage are as follows:

[0031]

[0032] 2. Experimental animals: healthy New Zealand white rabbits.

[0033] 3. Grouping of experimental animals: 6 gro...

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Abstract

The invention discloses a construction method of a drug evaluation model for dermal pathology of a tuberculosis rabbit. The method comprises: selecting a rabbit, injecting an immune drug into rabbit, conducting BCG (Bacillus Calmette Guerin) vaccine immune injection on the 15th to 20th day of drug intervention, and carrying out bacterial and pathological examination so as to construct a pathological model. The method of the invention establishes a pathological model of drug intervention on drug cutaneous tuberculosis with obvious symptoms so as to conduct visual research on tuberculosis pathology and bacterial pathogenicity, thus providing a visual animal model for vaccines and screening of drugs treating a tuberculosis necrotic liquefied cavity. And the model is stable and repeatable. The invention establishes a drug intervention procedure and observation indexes for research of drug intervention on a tuberculosis granuloma liquefaction process, and also provides a research basis for probing an immune mechanism about the formation of a tuberculosis liquefied cavity.

Description

technical field [0001] The invention relates to a method for constructing a disease animal model, in particular to a method for constructing a tuberculosis rabbit skin pathological drug evaluation model, which belongs to the medical field. Background technique [0002] Tuberculosis is a disease closely related to systemic immune status, but it mainly manifests as a local pathological injury. As the problem of tuberculosis drug resistance becomes more and more serious, the traditional anti-tuberculosis chemical drug treatment against Mycobacterium tuberculosis is no longer effective for some diseases, and people urgently need to develop new drugs to treat tuberculosis. Tuberculosis caseous necrosis and liquefaction and cavitation formation are dangerous pathological changes characteristic of human pulmonary tuberculosis and often become the source of dissemination. The liquefaction of necrotic tissue is a key link in the development of human pulmonary tuberculosis to the mal...

Claims

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Application Information

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IPC IPC(8): A61K39/04A61K45/00A61K38/20A61K38/21A61K31/675A61K31/573
Inventor 祝秉东张颖孙虹佳章国平马兴铭唐克峰达泽蛟雒艳萍
Owner LANZHOU UNIVERSITY
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