Special primer for assisting identification of carboxy-lesterase allele carried by culex mosquitoes and application thereof

Abstract

The invention discloses a special primer for assisting identification of a carboxy-lesterase allele carried by culex mosquitoes and application thereof. According to shown carboxy-lesterase allele, the invention designs a special primer consisting of an esterase B primer pair and an esterase A specific primer pair. The esterase B specific primer pair comprises a DNA shown in a sequence 1 of the sequence table and a DNA shown in a sequence 2 of the sequence table; the esterase A comprises a DNA shown in a sequence 3 of the sequence table and a DNA shown in a sequence 4 of the sequence table. APCR-RFLP method can be used to distinguish carboxy-lesterase allele by employing the special primer; a variety of a carboxy-lesterase in a wild mosquito can be determined to further calculate appearance frequency of the mosquito in a population through a macrorestriction map (in order to enhance accuracy, the macrorestriction map can be compared with a macrorestriction map of a known carboxy-lesterase resistance allele), so as to finally provide molecule basis for generation and development of accurate mosquito drug resistance.

Description

technical field [0001] The invention relates to a special primer for assisting the identification of carboxylesterase alleles carried by Culex mosquitoes and its application. Background technique [0002] Mosquitoes, due to their special behavior and physiological characteristics, have become the vectors of various diseases, which have seriously affected the tourism and catering industries, restricted the development of sanitary cities, and seriously endangered human health. There are many kinds of mosquitoes, and they transmit many diseases through their bites. Culex is the main vector of filariasis, Japanese encephalitis, and West Nile virus. Anopheles is the main vector of filariasis and malaria. Aedes mosquitoes It is the main transmission medium of dengue fever and Japanese encephalitis (Hemingway J and Ranson H.Insecticide resistance in insect vectors of humandisease[J].Annu.Rev.Entomol.2000,45:371-391.). [0003] At present, physical control, chemical control and bio...

AI Technical Summary

Problems solved by technology

The advantage of this method is that the accurate value of carboxylesterase activity can be obtained, but the disadvantage is that it must be completed with the aid of a microplate reader, which is troublesome in daily life

Another method is the filter paper method (O.Dary, G.P.Georghiou, E.Parsons and N.Pasteur: J.Econ.Entomol.1991, 84, 28-33.), the advantage of this method is that it can preserve the experiment for a long time As a result, the disadvantage is that only color reaction can be carried out on the filter paper, and the resistance level cannot be obtained due to the lack of sensitive strains as controls.

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