Method for extracting rivularinin from brook anemone

A technology of plum saponin and saxiflora, which is applied in the field of preparation of plum saponin, to achieve the effects of large-scale production operation, low energy consumption and low pollution

Inactive Publication Date: 2012-06-13
SUZHOU PAITENG BIOLOGICAL MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is no report on the preparation process applicable to the industrialized large-scale production of high-purity grass yumei saponin

Method used

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  • Method for extracting rivularinin from brook anemone

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Embodiment 1

[0023] Get 10Kg of tiger's palm root, pulverize, add to CO 2 In the supercritical extractor, ethanol is used as an entrainer, the entrainer accounts for 4% by volume of the total extraction solvent, the extraction pressure is 20MPa, the temperature is 45°C, CO 2 The flow rate is 1ml / g crude drug·min, the extraction time is 140min, and the extract is obtained, dissolved in water, and subjected to 7-stage countercurrent extraction with water-saturated n-butanol, and the n-butanol layer is taken, and the solvent is recovered under reduced pressure and concentrated, and added to D101 macroporous adsorption resin On the column, elute with 35% ethanol, collect 3 times the column volume of the eluate, recover the ethanol under reduced pressure and concentrate, add methanol to crystallize, separate the crystals, wash and dry to obtain 0.6g of osmanthus saponin, which is detected by HPLC , with a purity of 96.2%, UV, IR, MS, 2 HNMR, 13 The data characterizing its physical properties ...

Embodiment 2

[0025] Get 10Kg of tiger's palm root, pulverize, add to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer to the total extraction solvent is 6%, the extraction pressure is 30MPa, the temperature is 55°C, CO 2 The flow rate is 3ml / g crude drug·min, the extraction time is 160min, and the extract is obtained, dissolved in water, and subjected to 9-stage countercurrent extraction with water-saturated n-butanol, and the n-butanol layer is taken, and the solvent is recovered under reduced pressure and concentrated, and added to D101 macroporous adsorption resin On the column, elute with 35% ethanol, collect 8 times the column volume of the eluate, recover the ethanol under reduced pressure and concentrate, add methanol to crystallize, separate the crystals, wash and dry to obtain 0.8g of osmanthus saponin, which is detected by HPLC , with a purity of 95.4%, UV, IR, MS, 2 HNMR, 13 The data characterizing its physical pr...

Embodiment 3

[0027] Get 10Kg of tiger's palm root, pulverize, add to CO 2 In the supercritical extractor, ethanol is used as the entrainer, the entrainer accounts for 5% by volume of the total extraction solvent, the extraction pressure is 25MPa, the temperature is 50°C, CO 2 The flow rate is 2ml / g crude drug·min, the extraction time is 150min, and the extract is obtained, dissolved in water, and subjected to 8-stage countercurrent extraction with water-saturated n-butanol, and the n-butanol layer is taken, and the solvent is recovered under reduced pressure and concentrated, and added to D101 macroporous adsorption resin On the column, elute with 35% ethanol, collect the eluate with 5 times the volume of the column, recover the ethanol under reduced pressure and concentrate, add methanol to crystallize, separate the crystals, wash and dry to obtain 0.7g of osmanthus saponin, which is detected by HPLC , with a purity of 98.8%, UV, IR, MS, 2 HNMR, 13 The data characterizing its physical p...

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Abstract

The invention relates to a method for extracting rivularinin from brook anemone, which is simple and convenient to operate and has less pollution and less energy consumption. The method comprises the following process steps of: taking brook anemone roots, crushing and adding into a CO2 supercritical extractor; taking ethanol as an entrainer and obtaining an extract; adding water to dissolve and carrying out seventh-ninth backflow extraction by using water-saturated n-butyl alcohol, taking an n-butyl alcohol layer, depressurizing and recovering a solvent and concentrating; adding into a D101 macroporous adsorption resin column, eluting by using 35 percents of ethanol, collecting an eluent, depressurizing and recovering the ethanol and concentrating; and adding into methanol for crystallization, carrying out fractional crystallization, washing and drying to obtain the anemone rivularis saponin. The rivularinin prepared by the method has high product purity and easily realizes the industrialization amplification.

Description

technical field [0001] The invention relates to a preparation method of jasmine saponin, in particular to a preparation method of extracting sycamore saponin from plants. Background technique [0002] Rivularinin, molecular formula: C 53 h 84 o 22 , Molecular weight: 1073.233, CAS accession number: 70279-85-5, exists in a variety of plants, among which the Ranunculaceae plant Sagittarius Anemone rivularis It is abundant in the root of Buch.-Ham. ex DC. Its molecular formula is as follows. [0003] [0004] Modern research shows that japonica has antibacterial effect, and it is also used as a raw material for the synthesis of other active derivatives. [0005] Ranunculaceae Sagittarius Anemone rivularis The root of Buch.-Ham. ex DC. is used as a traditional Chinese medicine, which can clear away heat and detoxify, activate blood and relax tendons, reduce swelling and relieve pain. [0006] In the prior art, there is no report on the preparation process applicable to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
CPCY02P20/54
Inventor 王峰王琳张发成
Owner SUZHOU PAITENG BIOLOGICAL MEDICAL TECH
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