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Method for carrying out anesthesia and blood drawing on Takifugu obscurus

A technology of fugu obscura and anesthetics, applied in the field of aquaculture, can solve the problems of difficulty in finding the tail vein, high body fat content, and easy injury of the fish body, and achieve low cost, large amount of blood drawn, and strong operability Effect

Inactive Publication Date: 2012-07-04
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because puffer obscurus has a smooth body surface without scales, high body fat content and fat body, the fish body struggles when blood is drawn. It is not only difficult to find the tail vein with conventional tail vein blood collection, but also the fish body is extremely easy to be injured. Therefore, it is urgent to find a An Efficient Blood Collection Method for Fugu obscurus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A kind of anesthesia blood drawing method of fugu obscurus of the present invention adopts following operation steps:

[0018] First add pond water to the 80*80*60cm plastic holding tank, adjust the water temperature to 15-17°C, add commercially available clove oil (the concentration of eugenol is greater than 85%) to the holding tank, and prepare water and clove oil The weight ratio is 99.98:0.02 anesthetic solution; put 12 pufferfish obscurus with a weight of 110-120g into the anesthetic solution, anesthetize for 3-3.5 minutes, and you can see puffer obscura sideways in the water; quickly put pufferfish obscura Fish were fished out from the anesthesia solution, and blood was drawn from the tail vein with a disposable sterilized syringe with a capacity of 2ml. The blood drawing time was 1 minute, and the blood drawing volume was 1ml; After ~7 minutes, pufferfish obscura returned to normal swimming.

Embodiment 2

[0020] A kind of anesthesia blood drawing method of fugu obscurus of the present invention adopts following operation steps:

[0021] First add pond water to a plastic holding tank of 80*80*60cm, adjust the water temperature to 16-18°C, add commercially available clove oil (the concentration of eugenol is greater than 85%) to the holding tank, and prepare water and cloves The oil weight ratio is 99.98:0.02 anesthetic solution; put 10 pufferfish obscurus with a body weight of 120-130g into the anesthetic solution, anesthetize for 3.5-4 minutes, it can be seen that puffer obscura is poured sideways into the water; Fish fish were removed from the anesthesia solution, and blood was drawn from the tail vein with a disposable sterilized syringe with a capacity of 2ml. The blood drawing time was 1 minute, and the blood drawing volume was 1ml; After ~6 minutes, pufferfish obscura returned to normal swimming.

Embodiment 3

[0023] A kind of anesthesia blood drawing method of fugu obscurus of the present invention adopts following operation steps:

[0024] First add pond water to a plastic holding tank of 80*80*60cm, adjust the water temperature to 16-19°C, add commercially available clove oil (the concentration of eugenol is greater than 85%) to the holding tank, and prepare water and cloves The oil weight ratio is 99.98:0.02 anesthetic solution; put 8 pufferfish obscura with a body weight of 130-150g into the anesthetic solution, anesthetize for 4-5 minutes, and you can see puffer obscura sideways in the water; The pufferfish was taken out from the anesthesia solution, and blood was drawn from the tail vein with a disposable sterilized syringe with a capacity of 2ml. The blood drawing time was 1 minute, and the blood drawing volume was 1.5ml; after the blood drawing, the fish was transferred to the holding pond , 4 to 6 minutes later, Puffer obscurus returned to normal swimming.

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Abstract

The invention discloses a method for carrying out anesthesia and blood drawing on Takifugu obscurus, which comprises the following operating steps of: firstly, adding pood water into a container; regulating the water temperature into 15 to 20 DEG C; adding clove oil into the container to prepare anesthetic solution with the weight ratio of the water to the clove oil of 99.98:0.02; placing the Takifugu obscurus into the anesthetic solution, wherein the anesthesia time is 2 to 5 minutes; drawing blood by a syringe at the caudal vein, wherein the blood drawing time is 1 to 1.2 minutes; and after drawing blood, transferring the Takifugu obscurus into a temporary culturing pood for revival. According to the invention, aiming at the physiological property of the Takifugu obscurus, the method is successfully used for carrying out anesthesia and blood drawing on the Takifugu obscurus, has low cost, is simple and convenient to operate, has small damage to the fish body and has a large blood drawing quantity.

Description

technical field [0001] The invention relates to an anesthetized and blood-drawing method for puffer puffer, which belongs to the technical field of aquaculture. Background technique [0002] Fugu obscurus ( Takifugu Obscurus ), commonly known as puffer, belongs to Osteichthyes (Osteichthyes), Tetraodontiformes (Tetraodontiformes), Tetraodontidae (Tetraodontidae) Fugu (Fugu), is a migratory fish in rivers and seas, known as the "king of fish" in the world; from The tetrodotoxin extracted from its viscera is a deadly biological toxin, and it is also a powerful anesthetic and detoxification drug with high economic value. Due to the good market prospect, artificial breeding of puffer fish has developed rapidly since the success of artificial breeding in the 1990s. In recent years, due to the increasingly serious problems of germplasm degradation and germplasm mixing caused by inbreeding, artificial introduction and irregular multiplication and release, the research on the gene...

Claims

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Application Information

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IPC IPC(8): A01K61/00G01N1/14
CPCY02A40/81
Inventor 张敏莹施炜纲段金荣刘凯徐东坡周彦锋
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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