Liquid for preserving umbilical cord tissue

An umbilical cord tissue and liquid technology, applied in the field of biomedicine, can solve the problems of high price, high use cost, complicated PBS preparation, etc.

Active Publication Date: 2012-07-11
SICHUAN NEO LIFE STEM CELL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complicated preparation of PBS, a large amount of work will be added to the large-scale prep

Method used

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  • Liquid for preserving umbilical cord tissue
  • Liquid for preserving umbilical cord tissue
  • Liquid for preserving umbilical cord tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Under sterile conditions, the penicillin-streptomycin produced by the HYCLONE company filtered through a 0.22um filter membrane was prepared with sodium chloride injection (produced by Sichuan Kelun Pharmaceutical Co., Ltd.) to a concentration of 100 U of penicillin per milliliter and Streptomycin 100mg mixed solution), then aseptically dispensed into sterilized collection bottles, 150ml per bottle.

Embodiment 2

[0021] Take the preservation liquid of the above-mentioned embodiment 1 of the present invention, respectively carry out antibacterial effect test and umbilical cord growth test after storage. Then, the antibacterial effect test and the growth test of the umbilical cord after storage were carried out, and the parallel experiment was compared with the traditional PBS preservation solution. The preparation method of the traditional PBS preservation solution used for comparison:

[0022] PBS solvent composition: NaCl 8.0g

[0023] KCl0.2g

[0024] Na 2 HPO 4 12H 2 O 2.9g

[0025] K H 2 PO 4 0.2g

[0026] Ultrapure water to 1000ml.

[0027] PBS preservation solution composition: PBS sterilization solvent 990ml

[0028] Penicillin-streptomycin mixed solution 10ml

[0029] "PBS+double antibody" preservation solution 1000ml.

[0030] Preparation method of...

Embodiment 3

[0043] Corresponding preservation liquids were prepared in the same manner as in Example 1 and Example 2, and growth experiments after umbilical cord storage were carried out. The difference between the preservation liquid and Example 1 is that the contents of the penicillin-streptomycin in the penicillin-streptomycin mixture used are respectively 50 U-50 mg. The state of msc prepared by umbilical cord separation within 24 hours of collecting the umbilical cord in this preservation solution is as follows: Figure 7 shown.

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Abstract

The invention relates to a liquid for preserving an umbilical cord tissue, wherein the liquid is a solution, per ml of physiological saline contains 50-150 U of penicillin and 50-150 mg of streptomycin to form the solution. The preservation liquid has characteristics of wide source, stable quality, and cheap price. The experiment results show that: the liquid of the present invention provides the same effects in bacteriostasis property, effective preservation for the sampled umbilical cord tissue, and other fields as the traditional PBS preservation source, and the validity duration of the preservation liquid of the present invention is at least half a year, and can be prolonged.

Description

technical field [0001] The invention relates to a preservation liquid for preserving biological tissue components in the field of biomedicine, more specifically a preservation liquid for preserving umbilical cord tissue components. Background technique [0002] High-quality mesenchymal stem cells (MSCs) can be isolated from human umbilical cord tissue. MSCs are multipotent adult stem cells with self-renewal and multiple differentiation potentials, which have great application potential, for example, they can provide a suitable microenvironment for hematopoietic stem cells (HSCs), prevent and treat allogeneic hematopoietic stem cell transplantation Graft-versus-host disease (GVHD), as well as the treatment of autoimmune diseases such as lupus erythematosus, rheumatoid arthritis and scleroderma. [0003] In the process of MSCs reaching clinical application cells through in vitro culture and expansion, the tissues / cells need to be in a sterile state during the whole process of...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 任莹莹王荣邓云涛
Owner SICHUAN NEO LIFE STEM CELL BIOTECH
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