Preparation of conventional Pu'er tea microbial colony seeds and application of microbial colony seeds in Pu'er tea fermentation
A technology of microorganisms and microbial flora, applied in the directions of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of long fermentation period, incomplete flavor, and many miscellaneous bacteria, so as to overcome the long fermentation period, shorten the fermentation time, Overcome the effect of lack of flavor
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 Traditional naturally fermented high-quality big-leaf tea [Camellia sinersis (L.) O. Kuntze var. assamica (Masters) Kitamura] Pu-erh tea is isolated from its microbiota, preparation of "traditional Pu-erh tea microbiota seeds" and its fermentation of Pu-erh tea.
 A) Isolation of Pu'er tea flora: Take about 2g of traditional high-quality Pu'er tea that has just been produced by natural fermentation of big leaf tea, add it to a triangular flask filled with 18mL of pre-sterilized normal saline, shake it for 20 minutes, and make 10 -1 Microbial diluent; take 1 ml of the above 10 -1 Microbial dilution supernatant, added in 9 ml of pre-sterilized normal saline and shaken to make 10 -2 diluent; then take 1 ml of the above 10 -2 Microbial dilution, add to 9 ml of pre-sterilized normal saline and shake well to make 10 -3 diluent; and so on, to prepare 10 -1 to 10 -8 Dilutions of naturally fermented traditional Pu-erh tea microbial flora.
Take 0.1-0.2 millil...
 Isolation of microbiota from traditional naturally fermented high-quality Dali tea [C. taliensis (W.W.Smith) Melchior] Pu-erh tea, preparation of "traditional Pu-erh tea microbiota seeds" and fermentation of Pu-erh tea.
 A) Isolation of Pu'er tea flora: Completely according to the method for isolating its microbial flora from traditional natural fermented high-quality large-leaf tea Pu'er tea in Example 1, from traditional naturally fermented high-quality Dali tea Pu'er tea to isolate its microbial flora As a result, the microbial flora of traditional Dali tea and Pu'er tea 10 -6 22 single bacterium colonies appeared in the petri dish cultivated by the dilution liquid; its 22 single bacterium colonies were streaked and cultured and isolated in a petri dish filled with solid medium respectively to obtain pure strains, which were preserved for later use; the morphology observation and Physiological and biochemical tests showed that one of the colonies was harmful ...
 Separation of microbiota from traditional naturally fermented high-quality camellia (wild tea) Pu-erh tea, preparation of "traditional Pu-erh tea microbiota seeds" and fermentation of Pu-erh tea.
 A) Isolation of bitter tea Pu'er tea flora: fully follow the method of isolating its microbial flora from traditional natural fermented high-quality large-leaf tea Pu'er tea in Example 1, and isolate its microbial flora from traditional naturally fermented high-quality camellia Pu'er tea Cluster results, traditional camellia Puerh tea microbial flora 10 -5 33 single colonies and a combination of two colonies appeared in the culture dish of the dilution solution; the 33 single colonies and the combination of colonies were respectively streaked and cultured in a petri dish filled with solid medium to obtain 35 pure bacteria The morphological observation and physiological and biochemical tests of each colony microscope show that there are no harmful colonies, 11 colonies ...
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