Preparation of conventional Pu'er tea microbial colony seeds and application of microbial colony seeds in Pu'er tea fermentation

A technology of microorganisms and microbial flora, applied in the directions of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of long fermentation period, incomplete flavor, and many miscellaneous bacteria, so as to overcome the long fermentation period, shorten the fermentation time, Overcome the effect of lack of flavor

Active Publication Date: 2012-07-11
TIANJIN TASLY GROUP
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the technical problems existing in the existing fermentation process, the present invention proposes a method for preparing a novel "traditional Pu'er tea microbiota seed", and inoculates the Pu'er tea microbiota seed into raw green tea for fermentation, stable The production of high-quality traditional Puer tea; because the "traditional Puer tea microbiota seed" contains all the microbial flora fermented by traditional Puer tea, the quality of Puer tea fermented by it is the same as

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Traditional naturally fermented high-quality big-leaf tea [Camellia sinersis (L.) O. Kuntze var. assamica (Masters) Kitamura] Pu-erh tea is isolated from its microbiota, preparation of "traditional Pu-erh tea microbiota seeds" and its fermentation of Pu-erh tea.

[0027] A) Isolation of Pu'er tea flora: Take about 2g of traditional high-quality Pu'er tea that has just been produced by natural fermentation of big leaf tea, add it to a triangular flask filled with 18mL of pre-sterilized normal saline, shake it for 20 minutes, and make 10 -1 Microbial diluent; take 1 ml of the above 10 -1 Microbial dilution supernatant, added in 9 ml of pre-sterilized normal saline and shaken to make 10 -2 diluent; then take 1 ml of the above 10 -2 Microbial dilution, add to 9 ml of pre-sterilized normal saline and shake well to make 10 -3 diluent; and so on, to prepare 10 -1 to 10 -8 Dilutions of naturally fermented traditional Pu-erh tea microbial flora.

[0028]Take 0.1-0.2 millil...

Embodiment 2

[0039] Isolation of microbiota from traditional naturally fermented high-quality Dali tea [C. taliensis (W.W.Smith) Melchior] Pu-erh tea, preparation of "traditional Pu-erh tea microbiota seeds" and fermentation of Pu-erh tea.

[0040] A) Isolation of Pu'er tea flora: Completely according to the method for isolating its microbial flora from traditional natural fermented high-quality large-leaf tea Pu'er tea in Example 1, from traditional naturally fermented high-quality Dali tea Pu'er tea to isolate its microbial flora As a result, the microbial flora of traditional Dali tea and Pu'er tea 10 -6 22 single bacterium colonies appeared in the petri dish cultivated by the dilution liquid; its 22 single bacterium colonies were streaked and cultured and isolated in a petri dish filled with solid medium respectively to obtain pure strains, which were preserved for later use; the morphology observation and Physiological and biochemical tests showed that one of the colonies was harmful ...

Embodiment 3

[0049] Separation of microbiota from traditional naturally fermented high-quality camellia (wild tea) Pu-erh tea, preparation of "traditional Pu-erh tea microbiota seeds" and fermentation of Pu-erh tea.

[0050] A) Isolation of bitter tea Pu'er tea flora: fully follow the method of isolating its microbial flora from traditional natural fermented high-quality large-leaf tea Pu'er tea in Example 1, and isolate its microbial flora from traditional naturally fermented high-quality camellia Pu'er tea Cluster results, traditional camellia Puerh tea microbial flora 10 -5 33 single colonies and a combination of two colonies appeared in the culture dish of the dilution solution; the 33 single colonies and the combination of colonies were respectively streaked and cultured in a petri dish filled with solid medium to obtain 35 pure bacteria The morphological observation and physiological and biochemical tests of each colony microscope show that there are no harmful colonies, 11 colonies ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing conventional Pu'er tea microbial colony seeds and an application of the microbial colony seeds in Pu'er tea fermentation. The method comprises the following steps of: separating all microbial colonies from the high-quality Pu'er tea just produced by natural fermentation, and identifying the same bacteria and harmful bacteria; and removing the harmful bacteria, respectively culturing colonies except the harmful bacteria in a sterilized equal-weight tea or wheat-rice culture medium to obtain a pure strain culture, and performing inoculating fermentation and drying in the shade in green tea serving as a raw material to obtain high-quality Pu'er tea.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner TIANJIN TASLY GROUP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap