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Analytical method of nuclear texture of peripheral blood mononuclear cell

A texture analysis and nuclear cell technology, applied in the field of blood analysis, can solve problems such as loss, and achieve the effect of accurate and efficient classification and identification

Inactive Publication Date: 2012-07-11
ZHEJIANG OCEAN UNIV
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  • Application Information

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Problems solved by technology

Nowadays, with the widespread use of automated blood analyzers and the limitations of the human eye's resolution, the morphological examination of patients' peripheral blood cells (monocytes and other white blood cells) by artificial microscopes has gradually decreased, which may make us Information that is of great significance to the diagnosis of some diseases and the judgment of treatment effects is lost

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  • Analytical method of nuclear texture of peripheral blood mononuclear cell
  • Analytical method of nuclear texture of peripheral blood mononuclear cell
  • Analytical method of nuclear texture of peripheral blood mononuclear cell

Examples

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Embodiment 1

[0041] Example 1: Nuclei texture recognition of AIDS mononuclear cells.

[0042] The peripheral blood samples of AIDS patients were taken from the AIDS patients confirmed by the HIV diagnosis laboratory of Guizhou Provincial Center for Disease Control and Prevention. Peripheral blood cells were stained with methyl green-pyroline staining. Under the identification of the chief physician, the OLYMPUS CX31S photography microscope was used to capture the images of peripheral blood mononuclear cells of normal people and AIDS patients and store them in the computer hard disk. Peripheral blood mononuclear cell images of normal and AIDS patients such as Figure 1 to Figure 4 shown. Next, nuclear surface images were acquired, and nuclear images of size 40 pixels by 40 pixels were segmented from the nuclei of the captured mononuclear cell images. here, from figure 1The nuclear image of 40 pixels × 40 pixels is segmented from the nucleus of the cell nucleus, and the four nuclear imag...

Embodiment 2

[0047] Embodiment 2: Analysis of the relationship between surface texture of AIDS mononuclear cell nuclei image and viral load.

[0048] The measurable characteristics of the surface of the cell nucleus after chemical staining, such as smoothness, roughness, chromatin regularity, and chromatin texture directionality, are closely related to the changes of biological macromolecules such as nucleic acids and proteins inside the nucleus. That is to say, there is a certain correspondence between the surface features of the stained image of the nucleus and the internal substances of the nucleus. Use the 5 layers of coefficients after Curvelet transformation to reconstruct the feature image (gray image) such as Figure 13 ~ Figure 17 shown. Among them, (a) is the coefficient of the first layer, (b) is the coefficient of the second layer, (c) is the coefficient of the third layer, (d) is the coefficient of the fourth layer, (e) is the coefficient of the fifth layer, calculate the 5 l...

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Abstract

The invention relates to an analytical method of nuclear texture of peripheral blood mononuclear cell. The method comprises the following steps of: dying peripheral blood cells, obtaining a nuclear surface image of the dyed peripheral blood cells, performing curve wave transform to the nuclear surface image to obtain a coefficient matrix corresponding to image characteristics of the nuclear surface image, and finally performing transformation treatment to the efficient matrix to implement classification and identification of the nuclear surface image. Compared to the prior art, the method provided by the invention has the advantage that: pattern recognition combined with the curve wave transform is applied in the nuclear texture image analysis of the peripheral blood mononuclear cell to accurately and efficiently realize the classification and identification of normal cells with sick cells. The method has certain reference significance for realizing the rapid preliminary judgment of human immunodeficiency virus (HIV) loading capacity and the extent of damage of peripheral blood mononuclear cell under the condition of no expensive instruments and no agents.

Description

technical field [0001] The invention relates to a blood analysis method, in particular to a nuclear texture analysis method of peripheral blood mononuclear cells. Background technique [0002] One of the important directions of medical research is to conduct morphological research on various biological structures, especially quantitative morphological research. One of the quantitative morphological studies is to explore the changes of biological morphological structure and characteristics under pathological conditions. The most studied in the field of medical image recognition are image feature (morphological structure) extraction and target recognition. Under normal circumstances, after staining of various cells in blood, under the microscope, the nuclei of different types of cells have different nuclear texture morphological features, such as fine reticular, coarse reticular, tight chromatin, loose chromatin etc. It can be considered that the texture of the nucleus can ...

Claims

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Application Information

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IPC IPC(8): G01N33/49G06K9/62
Inventor 李大东杨兴林熊金凤
Owner ZHEJIANG OCEAN UNIV
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