AEG-1 (Astrocyte Elevated Gene-1)/1E3 monoclonal antibody with high affinity

Abstract

The invention discloses an AEG-1 (Astrocyte Elevated Gene-1) / 1E3 monoclonal antibody with high affinity. The amino acid sequence of a variable region of a light chain of AEG-1 / 1E3mAb is shown as SEQ ID. NO. 1, and the amino acid sequence of a variable region of a heavy chain is shown as SEQ ID. NO. 2. The uniqueness for the gene sequence and corresponding protein sequence of a variable region of the antibody and CDR (Complementary Determined Region) sequence of the antibody are determined; the AEG-1 / 1E monoclonal antibody has high affinity with AEG-1, and the AEG-1 can be specifically identified and immunological reaction can be performed; and the titer of ascites is 1*10<-7>. Support is provided for researching and developing anti-AEG-1 gene engineering antibody and a diagnostic reagent.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine and relates to a monoclonal antibody, in particular to an AEG-1 / 1E3 monoclonal antibody with high affinity, including its amino acid sequence and nucleotide sequence. Background technique

[0002] With the completion of the sequencing of the human genome, we are more aware that for the more than 30,000 functional genes in the human genome, we only understand the basic functions and functions of a very small number of genes, while more, unknown new genes and their The physiological and pathological roles played by the encoded proteins still need to be deeply explored and explored, especially various new genes closely related to the occurrence and development of malignant tumors and their functions have been gradually recognized and elucidated. Astrocyte up-regulated gene 1 (AEG-1) is a newly discovered important oncogene that regulates the biological phenotype of various tumor cells. Since thi...

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