AEG-1 (Astrocyte Elevated Gene-1)/1E3 monoclonal antibody with high affinity

A technology of AEG-1 and monoclonal antibody, which is applied in the field of biomedicine and can solve the inconvenience of in-depth research on AEG-1

Active Publication Date: 2012-07-18
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] AEG-1 variable region antibody is an indispensable tool in the research of the above problems. However, there are no research reports on the preparation of anti-AEG-1 variable region antibody at home and abroad, which brings great benefits to the in-depth study of AEG-1. many inconveniences

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  • AEG-1 (Astrocyte Elevated Gene-1)/1E3 monoclonal antibody with high affinity
  • AEG-1 (Astrocyte Elevated Gene-1)/1E3 monoclonal antibody with high affinity
  • AEG-1 (Astrocyte Elevated Gene-1)/1E3 monoclonal antibody with high affinity

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Embodiment Construction

[0032] The present invention immunizes BALB / c mice with natural AEG-1, prepares a group of hybridoma cell lines secreting mouse anti-human AEG-1 monoclonal antibody, and screens out AEG-1 which can stably secrete high-affinity anti-AEG-1. 1 / 1E3mAb hybridoma cell line, prepare ascites to obtain high-affinity anti-AEG-1 monoclonal antibody; use the hybridoma cells to extract total cellular RNA, and use degenerate primers to amplify the light and heavy chain variable regions of the antibody after reverse transcription Gene, and confirm the uniqueness of the gene sequence and corresponding amino acid sequence and its CDR sequence; provide support for anti-AEG-1 chimeric or humanized genetic engineering antibodies. The present invention will be described in detail below in conjunction with the preparation method of specific monoclonal antibodies, antibody activity detection, and sequence detection and uniqueness determination, which is an explanation rather than a limitation of the ...

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Abstract

The invention discloses an AEG-1 (Astrocyte Elevated Gene-1)/1E3 monoclonal antibody with high affinity. The amino acid sequence of a variable region of a light chain of AEG-1/1E3mAb is shown as SEQ ID. NO. 1, and the amino acid sequence of a variable region of a heavy chain is shown as SEQ ID. NO. 2. The uniqueness for the gene sequence and corresponding protein sequence of a variable region of the antibody and CDR (Complementary Determined Region) sequence of the antibody are determined; the AEG-1/1E monoclonal antibody has high affinity with AEG-1, and the AEG-1 can be specifically identified and immunological reaction can be performed; and the titer of ascites is 1*10<-7>. Support is provided for researching and developing anti-AEG-1 gene engineering antibody and a diagnostic reagent.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a monoclonal antibody, in particular to an AEG-1 / 1E3 monoclonal antibody with high affinity, including its amino acid sequence and nucleotide sequence. Background technique [0002] With the completion of the sequencing of the human genome, we are more aware that for the more than 30,000 functional genes in the human genome, we only understand the basic functions and functions of a very small number of genes, while more, unknown new genes and their The physiological and pathological roles played by the encoded proteins still need to be deeply explored and explored, especially various new genes closely related to the occurrence and development of malignant tumors and their functions have been gradually recognized and elucidated. Astrocyte up-regulated gene 1 (AEG-1) is a newly discovered important oncogene that regulates the biological phenotype of various tumor cells. Since thi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/32C12N15/13C12N15/63G01N33/577A61K39/395A61P35/00
Inventor 张惠中董轲龙敏陈曦
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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