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In-vitro rapid propagation method for notopterygium tissue culture

A technique of tissue culture and notopterygium, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem that wild notopterygium young leaves are explanted tissue culture has not been reported yet, and achieve good biological characteristics, induce The effect of promoting cell elongation and growth in a short period of time

Inactive Publication Date: 2012-09-12
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The literature has reported the tissue culture of many alpine wild medicinal materials, such as Saussurea chinensis, Rhodiola rosea, etc., but the tissue culture of wild notopterygia young leaves as explants has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 A method for in vitro rapid propagation of notopterygium tissue culture, comprising the following steps:

[0030] (1) Selection and disinfection of explants: Take the young leaves of notopterygium as explants, wash them with running water for 10 minutes, then sterilize them with alcohol with a volume concentration of 75% for 15 seconds, and then rinse them with sterile deionized water for 2 minutes. ~3 times, then place the washed explants in mercuric chloride with a mass concentration of 0.1%, soak and sterilize for 8 minutes, rinse with sterile deionized water for 3 times, and finally dry the water with sterile filter paper to obtain Sterilized explants.

[0031] ⑵Explant inoculation: Cut the sterilized explant into 0.3~0.5cm 2 The small pieces of the callus tissue were inoculated on the callus induction medium, wherein 3 small pieces of sterilized explants were inoculated in every 25ml of the callus induction medium; at a temperature of 20°C ± 2°C, a light...

Embodiment 2

[0042] Example 2 A method for in vitro rapid propagation of notopterygium tissue culture, comprising the following steps:

[0043] (1) Selection and disinfection of explants: Take the young leaves of notopterygium as explants, wash them with running water for 15 minutes, then soak them in alcohol with a volume concentration of 75% for 20 seconds, and then rinse them with sterile deionized water for 2 minutes. Then place the washed explants in mercuric chloride with a mass concentration of 0.2%, soak and sterilize for 6 minutes, rinse with sterile deionized water for 5 times, and finally dry the water with sterile filter paper to obtain the sterilized of explants.

[0044] ⑵Explant inoculation: Cut the sterilized explant into 0.3~0.5cm 2 The small pieces of the callus tissue were inoculated on the callus induction medium, wherein 3 small pieces of sterilized explants were inoculated in every 25ml of the callus induction medium; at a temperature of 20°C ± 2°C, a light source of...

Embodiment 3

[0055] Example 3 A method for in vitro rapid propagation of notopterygium tissue culture, comprising the following steps:

[0056] (1) Selection and disinfection of explants: Take the young leaves of Notopterygium explants as explants, rinse them with running water for 15 minutes, then soak them in 75% alcohol for 30 seconds, and then rinse them with sterile deionized water for 30 seconds. Then, place the washed explants in 0.15% mercuric chloride, immerse and sterilize for 7 minutes, rinse with sterile deionized water for 4 times, and finally dry the water with sterile filter paper to obtain the sterilized of explants.

[0057] ⑵Explant inoculation: cut the sterilized explant into 0.1~0.3cm 2 The small pieces of the callus tissue were inoculated on the callus induction medium, wherein 3 small pieces of sterilized explants were inoculated in every 25ml of the callus induction medium; at a temperature of 20°C ± 2°C, a light source of fluorescent lamps, and light intensity 30~...

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PUM

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Abstract

The invention relates to an in-vitro rapid propagation method for notopterygium tissue culture. The method comprises the following steps: (1) selection and sterilization on explants: based on spires of notopterygium as explants, carrying out soaking sterilization and washing on the spires so as to obtain sterilized explants; (2) explant inoculation: slicing the sterilized explants, and inoculating the sliced explants to a callus inducing medium so as to form calluses; (3) callus multiplication: carrying out multiplication on inoculated calluses so as to form dense calluses; (4) bud induction of calluses: carrying out inoculation on the dense calluses so as to form fasciculate notopterygium seedlings; (5) rooting induction: carrying out inoculated culture on the fasciculate notopterygium seedlings subjected to plant division so as to obtain complete regenerated plantlets; (6) carrying out sterilization on nursing mediums; and (7) plant transplanting: directly transplanting the complete regenerated plantlets to the sterilized nursing mediums, so that after exercising seedlings, the plantlets grow into normal notopterygium plants. The method disclosed by the invention solves the problem that the notopterygium is overlong in seedling growing period and low in propagation coefficient; the method is easy to operate; and the method can be applied to rapid seedling growing in factories.

Description

technical field [0001] The invention relates to tissue culture and in vitro rapid propagation of plants, in particular to a method for in vitro rapid propagation of notopterygium tissue culture. Background technique [0002] Notopterygium belongs to the genus Notopterygium (Umbelliferae) of the family Umbelliferae ( Notopterygium ) perennial plant is an important and endangered plant unique to my country. It is one of the most commonly used medicinal materials in Chinese, Tibetan and Qiang medicines, and it is also a Chinese and Tibetan medicinal material with a large market gap. Notopterygium is used as a medicine with rhizomes and roots. It is pungent in taste and warm in nature. Notopterygium is recorded in all previous editions of Chinese Pharmacopoeia. According to the 2005 edition of "Pharmacopoeia of the People's Republic of China", it is a perennial herb of Umbelliferae (Umbelliferae). Notopterygium incisum Ting ex H.T. Chang) and Qianghuo broad leaf ( N. forbes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 徐文华周国英刘卫根杨路存贺丽华李春丽
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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