Method for preparing esterified derivative of lily polysaccharide by biological catalysis

A lily polysaccharide and biocatalysis technology, which is applied in the production and fermentation of bulk chemicals, can solve problems such as complex structure, and achieve the effect of overcoming easy evaporation and reducing production costs.

Inactive Publication Date: 2012-09-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since lily polysaccharide is a high molecular compound (Mw 350.0kDa), main

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Put 1.0g lily polysaccharide, 30ml C 4 MIm·BF 4 Ionic liquid, 50 mg of lipase derived from Pseudomonas cepacia (Burkholderia cepacia) (2000 U / g, Japan Amano Enzyme Co., Ltd.) and 5 ml of vinyl acetate were placed in a stoppered Erlenmeyer flask, placed in a water bath at 35 °C and 200 rpm Shake in a constant temperature oscillator, react for 12 hours, filter to remove lipase, then add ethanol to produce a white precipitate, filter, wash thoroughly with ethanol, and vacuum dry (30°C, 0.05MPa) to obtain lily polysaccharide with a substitution degree of 0.23 Acetate Derivatives.

Embodiment 2

[0026] Put 1.0g lily polysaccharide, 70ml C 4 MIm·BF 4 Ionic liquid, 250mg of lipase (2000U / g, Japan Amano Enzyme Co., Ltd.) derived from Pseudomonas cepacia (Burkholderia cepacia) and 12ml of vinyl acetate were placed in a stoppered Erlenmeyer flask, placed in a water bath at 55°C and 200rpm Shake in a constant temperature oscillator, react for 24 hours, filter to remove lipase, then add ethanol to produce a white precipitate, filter, wash thoroughly with ethanol, and dry in vacuum (30°C, 0.05MPa) to obtain lily polysaccharide with a substitution degree of 0.71 Acetate Derivatives.

Embodiment 3

[0028] Put 1.0g lily polysaccharide, 50ml C 4 MIm·BF 4 Ionic liquid, 150 mg of lipase (2000 U / g, Japan Amano Enzyme Co., Ltd.) derived from Pseudomonas cepacia (Burkholderia cepacia) and 7 ml of vinyl butyrate were put into a stoppered Erlenmeyer flask, placed at 45 ° C, 200 rpm Shake in a constant temperature shaker in a water bath, react for 18 hours, filter to remove lipase, then add ethanol to produce a white precipitate, filter, wash thoroughly with ethanol, and dry in vacuum (30°C, 0.05MPa) to obtain a lily with a substitution degree of 0.42 Polysaccharide butylated derivatives.

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PUM

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Abstract

The invention belongs to the fields of biological catalysis and biotransformation and discloses a method for preparing an esterified derivative of lily polysaccharide by biological catalysis. The method comprises the following steps of catalyzing lily polysaccharide by utilizing lipase for transesterification reaction at the temperature of 35-55 DEG C based on an ionic liquid as a solvent and vinyl ester fatty acid with C2-C18 carbon chain length as an acyl group donor and synthesizing to obtain the esterified derivative of lily polysaccharide. The method has the advantages that reaction condition is moderate and environment-friendly, the reaction is controllable and simple in process, the product is easy to separate from a reaction mixing system, and the like.

Description

technical field [0001] The invention belongs to the field of biocatalysis and biotransformation, and relates to a method for preparing lily polysaccharide esterified derivatives by biocatalysis. Background technique [0002] Lily is one of the first batch of medicine and food approved by the Ministry of Health. It not only has a wide range of clinical applications, but also has a good health care effect. Lily polysaccharide is one of the main functional factors of lily. Lily polysaccharide is mainly composed of D-glucose, D-mannose and galactose combined by pyranoside bonds. Studies have found that: Lily polysaccharide has various biomedical functions such as anti-tumor, lowering blood fat, anti-virus, anti-mutation and enhancing immunity. Therefore, by modifying the structure of lily polysaccharide compounds, the research work of looking for more active lily polysaccharide derivatives has attracted much attention. [0003] At present, the synthesis of esterified derivativ...

Claims

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Application Information

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IPC IPC(8): C12P19/04
CPCY02P20/54
Inventor 陈志刚曹林顾振新韩永斌
Owner NANJING AGRICULTURAL UNIVERSITY
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