Novel I-type human immunodeficiency virus (HIV-1) infection enzyme test-free reagent kit

A technology for human immunodeficiency and HIV-1, applied in the field of immune detection kits, can solve the problem of high misjudgment rate

Inactive Publication Date: 2012-09-19
BEIJING KINGHAWK PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, only Sedia Company of the United States can produce similar products. The HIV-1 Emerging Infection Detection Kit (BED method) produced by this company has been widely used in the field of HIV-1 Emerging Infection Detection, but there are errors in the use of this method. high rate problem

Method used

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  • Novel I-type human immunodeficiency virus (HIV-1) infection enzyme test-free reagent kit
  • Novel I-type human immunodeficiency virus (HIV-1) infection enzyme test-free reagent kit
  • Novel I-type human immunodeficiency virus (HIV-1) infection enzyme test-free reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] 【Main components】

[0103]

[0104]

[0105] 【Storage conditions】

[0106] This kit is available in refrigerated and frozen packages.

[0107] Storage conditions in refrigerated packaging: 2-8°C

[0108] Storage conditions for frozen packages: ≤-20°C

[0109] 【Applicable instrument】

[0110] 1. Automatic microplate washing machine, microplate reader

[0111] 2. Pipette

[0112] 3. Oscillator

[0113] 4. Sample dilution tube

[0114] 5. Timer

[0115] 6. Thermostat (37°C and 25°C)

[0116] 【Sample Requirements】

[0117] 1. The applicable sample of this product is liquid serum or plasma.

[0118] 2. The test samples of this product are all HIV-1 positive samples.

[0119] 3. Samples should be stored at 2-8°C for no more than 2 weeks. Long-term storage must be at -20°C or below.

[0120] 4. Avoid repeated freezing and thawing of samples, and samples with severe hemolysis and microbial contamination cannot be used.

[0121] 5. Before use, the sample can ...

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PUM

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Abstract

The invention provides a novel I-type human immunodeficiency virus (HIV-1) infection enzyme test-free reagent kit. The reagent kit comprises A, HIV-1gp41 protein, B, a blank enzyme linked plate, C, acid eluent, D, goat anti-human immunoglobulin G (IgG) marked with horseradish peroxidase, E, tetramethyl benzidine (TMB) color development solutions and F, stop solutions.

Description

Technical field: [0001] The present invention relates to an immunoassay kit, its composition, preparation and application, in particular to an ELISA kit (restricted antigen affinity method) for type I human immunodeficiency virus (HIV-1) emerging infection and The preparation method thereof, the kit is mainly used for distinguishing HIV-1 recent infection and long-term infection at the population level. Background technique: [0002] Prospective cohort studies in epidemiological methods have always been considered the "gold standard" for detecting new HIV infections. However, cohort studies cannot be widely used due to the huge investment in manpower, time and funds. In addition, cohort studies are lost to follow-up, so semi-open or open cohorts have large changes in the monitoring population. Once the quality of the cohort cannot be controlled, the entire cohort will be biased or even lose representativeness. More importantly, during the cohort observation process, the fol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
Inventor 戈军叶艺张誌曹健荣
Owner BEIJING KINGHAWK PHARMA
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