Preparation method of enhanced Au (at) PtNPs probe and nano-enzyme test strip with enhanced Au (at) PtNPs probe

An enhanced nanozyme technology, applied in the field of medical devices, can solve the problems of insufficient sensitivity and stability, achieve the effect of improving sensitivity and stability, and strengthening the detection effect

Pending Publication Date: 2022-07-22
柳州康云互联科技有限公司
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing an enhanced Au@PtNPs probe and a nanozyme test strip with an enhanced Au@PtNPs probe, aiming at solving the problems of insufficient sensitivity and stability

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  • Preparation method of enhanced Au (at) PtNPs probe and nano-enzyme test strip with enhanced Au (at) PtNPs probe

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Embodiment Construction

[0016] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0017] like figure 1 , to prepare Au@PtNPs solution, add 200 mL of water into a conical flask, add 200 μL of 10% chloroauric acid, shake and mix well, heat the solution on an electric furnace until the solution boils, and quickly add 3 mL of 1% trisodium citrate , keep shaking so that the solution is in a slightly boiling state, continue to heat for about 15min to ensure that the reduction reaction is fully carried out, then add water to 200mL, continue to heat the colloidal gold solution, maintain a slight boiling state, first add ascorbic acid, and then add chloroplatinic acid for 15min , to ensure that the reduction reaction is fully carried out, then add water to 200 mL, and cool to room temperature to obtain an Au@PtNPs solution for later use.

[0018] To prepare biotinylated antibody, dilute the antibody with 0.1M sodium bicarbonate buffer...

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Abstract

The invention relates to a preparation method of an enhanced Au (at) PtNPs probe and a nano-enzyme test strip with the enhanced Au (at) PtNPs probe. The preparation method of the enhanced Au (at) PtNPs probe comprises the following steps: (1) preparing an Au (at) PtNPs solution; (2) preparing a biotinylated antibody; and (3) preparing the enhanced Au (at) PtNPs probe. Avidin is combined into the Au (at) PtNPs probe by utilizing an innovative preparation method, so that the sensitivity and the stability of the Au (at) PtNPs probe are remarkably improved, and then the Au (at) PtNPs probe is utilized to prepare the nano-enzyme test strip with the enhanced Au (at) PtNPs probe, so that the detection effect can be enhanced, and a key effect can be achieved on strict investigation of new crown epidemic situations.

Description

【Technical field】 [0001] The invention relates to the field of medical devices, in particular to a preparation method of an enhanced Au@PtNPs probe and a nano-enzyme test strip with the enhanced Au@PtNPs probe. 【Background technique】 [0002] Au@PtNPs probes have a variety of existing preparation methods. Nanozyme detection test strips also use Au@PtNPs probes as their components, but their sensitivity and stability in response to epidemic detection, especially early investigation, still need to be promote. [Content of the invention] [0003] The purpose of the present invention is to provide a preparation method of an enhanced Au@PtNPs probe and a nanoenzyme test strip with an enhanced Au@PtNPs probe, aiming at solving the problems of insufficient sensitivity and stability. [0004] The present invention is realized as follows: a method for preparing an enhanced Au@PtNPs probe, comprising the following steps: [0005] (1) Prepare the Au@PtNPs solution, add water to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 吴凡司朝朝张大兵颜光涛
Owner 柳州康云互联科技有限公司
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