Sex pheromone of trichophysetis cretacea and lure thereof
A technology of sex and pheromone of C. bistritus, which is applied in the field of sex pheromone of C. bistritus and its related lures, can solve the problems of no identification of chemical structure, etc.
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[0021] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0022] 1. Extraction of sex pheromones
[0023] Collect aged or mature larvae, rear them in the laboratory, and prepare them after eclosion; use sexually mature unmated female moths, aged 3-5 days after eclosion. Extraction time is 6-7 hours after dark at night. Take adult moths, after ether anesthesia, gently press the abdomen with medical surgical tweezers, then cut off the 3 segments at the end of the abdomen, and transfer them into a clean glass vial with about 100ul. After leaching for 10 minutes, draw the solution part into the pointed bottom glass tube , concentrated under a slight nitrogen atmosphere, and analyzed by gas phase-mass spectrometry.
[0024] 2. Identification and synthesis
[0025] Gas phase analysis conditions: All synthetic sex pheromone compounds were analyzed on Agilent 6890GC-5975MS, the chromatographic column was ...
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