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Application of amino acid ionic liquid as ligand for zinc complex in chiral separation by capillary electrophoresis

A technology of capillary electrophoresis and coordination compounds, which is applied in the field of chiral separation in analytical chemistry, and achieves the effects of wide application prospects, strong chiral recognition ability, and simple operation

Inactive Publication Date: 2012-10-24
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, amino acid ionic liquids have not been complexed with zinc ions as a new chiral selector in chiral ligand exchange capillary electrophoresis for chiral resolution of derivatized amino acids

Method used

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  • Application of amino acid ionic liquid as ligand for zinc complex in chiral separation by capillary electrophoresis
  • Application of amino acid ionic liquid as ligand for zinc complex in chiral separation by capillary electrophoresis

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Taking the mixed sample of chiral amino acid dansyl chloride derivatized isoleucine (Ile), dansyl chloride derivatized methionine (Met) and dansyl chloride derivatized serine (Ser) as an example, first configure the electrophoresis buffer as the background electrolyte , consisting of 100.0mM (the concentration is the final concentration in the background electrolyte) sodium borate, 5.0mM (the concentration is the final concentration in the background electrolyte) ammonium acetate, 3.0mM (the concentration is the final concentration in the background electrolyte) Zinc sulfate, 6.0 mM (the concentration is the final concentration in the background electrolyte) 1-hexyl-3-methylimidazolium L-lysine salt, buffer solution of water adjusted to pH with solid Tris 8.2, filtered through a 0.45 μm microporous membrane, and degassed by ultrasonic for later use. First wash the capillary with 1.0M NaOH aqueous solution, 1.0M HCl solution and water for half an hour, then wash the capi...

Embodiment 2

[0026]Mixed samples of chiral amino acid dansyl chloride derivatized threonine (Thr), dansyl chloride derivatized phenylalanine (Phe), dansyl chloride derivatized tyrosine (Tyr) and dansyl chloride derivatized asparagine (Asn) As an example, first configure the electrophoresis buffer as the background electrolyte: 100.0mM (the concentration is the final concentration in the background electrolyte) sodium borate aqueous solution, 5.0mM (the concentration is the final concentration in the background electrolyte) ammonium acetate aqueous solution, 3.0 Composition of mM (the concentration is the final concentration in the background electrolyte) zinc sulfate aqueous solution, 6.0 mM (the concentration is the final concentration in the background electrolyte) 1-hexyl-3-methylimidazole L-lysine salt solution and water The buffer solution was adjusted to pH 8.2 with solid tris, filtered through a 0.45 μm microporous membrane, and ultrasonically degassed for later use. First wash the ...

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Abstract

The invention discloses a coordination compound with amino acid ionic liquid as a ligand for a zinc complex, and a preparation method and application thereof. The coordination compound provided in the invention comprises central ions and ligands, wherein the central ions are zinc ions and the ligands are 1-hexyl-methylimidazole L-lysinate. According to the invention, amino acid ionic liquid with L-lysine as anions is used as a novel ligand for the zinc complex in chiral ligand exchange capillary electrophoresis, and therefore, a novel chiral resolution method is established. The ligand has the advantages of good chiral characteristics, low cost and environment friendliness, and the established chiral resolution method is simple to operate and has strong chiral recognition capability and wide application prospects.

Description

technical field [0001] The invention belongs to the field of analytical chemistry chiral separation, and relates to the application of an amino acid ionic liquid as a ligand of a zinc complex in capillary electrophoresis chiral separation. Background technique [0002] Chiral recognition has attracted extensive attention due to its important biochemical significance, and has triggered the rapid development of various chiral resolution methods. Among them, capillary electrophoresis is considered to be a high-efficiency separation technique that can be used for chiral resolution due to its outstanding advantages such as high separation efficiency, small sample consumption and simple operation. Currently, chiral resolution in capillary electrophoresis is mainly achieved through the interaction between the analyte and various chiral selectors. Commonly used chiral selectors include: cyclodextrins, macrocyclic antibiotics, polysaccharides, chiral crown ethers, proteins, and chir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F3/06G01N27/447
Inventor 齐莉张海枝木肖玉乔娟毛兰群
Owner INST OF CHEM CHINESE ACAD OF SCI
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