Tissue transparent reagent, and preparation method and applications thereof

Abstract

The invention discloses tissue transparent reagent, and a preparation method and applications thereof. The tissue transparent reagent comprises the following components in percentage by volume: 0.59 to 0.79 percent of a-pinene, 0.45 to 0.65 percent of b-pinene, 0.45 to 0.65 percent of limonene, 0.45 to 0.65 percent of terpene, 0.45 to 0.65 percent of longifolene, 0.45 to 0.65 percent of caryophyllene, 0.45 to 0.65 percent of aldehyde citral juice, 32.3 to 34.3 percent of d-limonene, 26.7 to 28.7 percent of gamma-terpinene, 0.45 to 0.65 percent of capric aldehyde, 0.45 to 0.65 percent of caprylic aldehyde, 0.45 to 0.65 percent of citral and 32.3 to 34.3 percent of normal butanol. The tissue transparent reagent can replace the conventional transparent reagent xylene, sample tissue treated by the tissue transparent reagent does not become fragile, and is good in the transparent effect.

Description

technical field

[0001] The invention relates to the field of tissue sample preparation, in particular to a tissue transparent reagent and its preparation method and application. Background technique

[0002] In medical and biological research, the transparency of biological tissue samples is helpful for doctors and scientific researchers to observe and study. In the field of transparent tissue sample preparation, the most widely used transparent agent is xylene. Xylene is a colorless, transparent, volatile liquid with an aromatic smell. It acts quickly and can be mixed with alcohol to dissolve paraffin. The gum used for sealing is mixed, but the water must be removed before use, otherwise milky turbidity will occur. In order to avoid material shrinkage, a gradual transition from pure alcohol to xylene should be adopted, that is, absolute alcohol → 1 / 2 absolute alcohol + 1 / 2 xylene → xylene. However, xylene is highly toxic and harmful to human health. It can enter the human...