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Tissue transparent reagent, and preparation method and applications thereof

A technology for transparent reagents and tissues, applied in the field of tissue transparent reagents and its preparation, can solve the problems of high toxicity of xylene and hazards to human health, achieve good transparent effect, rapid action, and solve safety and environmental protection problems

Inactive Publication Date: 2012-10-24
王虎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, xylene is highly toxic and harmful to human health. It can enter the human body through inhalation, ingestion, and percutaneous absorption.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0058] The preparation method of the tissue clearing reagent is provided in the present invention, which specifically includes the following steps:

[0059] According to the above formula, add each component into the container in turn, put the electromagnetic rod into the liquid, and stir it on the induction cooker for about 60 minutes until all the ingredients are dissolved to get the product.

[0060] The tissue clearing reagent can be used to replace the xylene clearing chemical reagent, which acts quickly, can be mixed with alcohol to dissolve paraffin, and can be mixed with gum for sealing. However, the tissue transparent reagent must be dehydrated during use, otherwise milky turbidity will occur. In order to avoid acute shrinkage of the material, the tissue-clearing reagent should gradually transition from pure alcohol to absolute alcohol → 1 / 2 absolute alcohol + 1 / 2 the tissue-clearing reagent → the tissue-clearing reagent.

[0061] The application of the tissue cleari...

Embodiment 1

[0079] According to volume percentage, take 25 ml of a-pinene, 20 ml of b-pinene, 20 ml of limonene, 20 ml of terpene, 20 ml of longifolene, 20 ml of caryophyllene, 20 ml of aldehyde citral juice, and 20 ml of d-taro 1200 ml of ene, 1000 ml of γ-terpinene, 20 ml of decanal, 20 ml of octanal, 20 ml of citral, and 1200 ml of n-butanol, add each component into the container in turn, put the electromagnetic rod into the liquid , Stir on the induction cooker for 60 minutes, until it is completely dissolved, the product can be obtained.

[0080] The dehydration process of the tissue-clearing reagent is as follows: absolute alcohol → 1 / 2 absolute alcohol + 1 / 2 the tissue-clearing reagent → the tissue-clearing reagent.

[0081] Take 33 organs such as rat brain, thyroid, lung, liver, and 22 kinds of human tissues. After the samples are soaked in alcohol, put them in the tissue transparent agent for 6 hours, then immerse them in 65°C wax liquid, and finally slice them. deal with. It h...

Embodiment 2

[0083] According to volume percentage, take a-pinene 15.8ml, b-pinene 9.2ml, limonene 11ml, terpene 11ml, longifolene 11ml, caryophyllene 11ml, aldehyde citral juice 9.2ml, d-tarone 686ml, 546ml of γ-terpinene, 9.2ml of decanal, 11ml of octanal, 9.2ml of citral, and 660.4ml of n-butanol. Add each component to the container in turn, put the electromagnetic rod into the liquid, and place it on the induction cooker. Stir it up for 60 minutes until it is completely dissolved to get this product.

[0084] The dehydration process of the tissue-clearing reagent is as follows: absolute alcohol → 1 / 2 absolute alcohol + 1 / 2 the tissue-clearing reagent → the tissue-clearing reagent.

[0085] Take 20 organs such as the rat brain, thyroid, lung, liver, etc. After the samples are soaked in alcohol, they are immersed in the tissue transparent agent for 6 hours, then immersed in 65°C wax solution, and finally sliced. It has been confirmed by sectioning that the tissue transparent reagent can...

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PUM

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Abstract

The invention discloses tissue transparent reagent, and a preparation method and applications thereof. The tissue transparent reagent comprises the following components in percentage by volume: 0.59 to 0.79 percent of a-pinene, 0.45 to 0.65 percent of b-pinene, 0.45 to 0.65 percent of limonene, 0.45 to 0.65 percent of terpene, 0.45 to 0.65 percent of longifolene, 0.45 to 0.65 percent of caryophyllene, 0.45 to 0.65 percent of aldehyde citral juice, 32.3 to 34.3 percent of d-limonene, 26.7 to 28.7 percent of gamma-terpinene, 0.45 to 0.65 percent of capric aldehyde, 0.45 to 0.65 percent of caprylic aldehyde, 0.45 to 0.65 percent of citral and 32.3 to 34.3 percent of normal butanol. The tissue transparent reagent can replace the conventional transparent reagent xylene, sample tissue treated by the tissue transparent reagent does not become fragile, and is good in the transparent effect.

Description

technical field [0001] The invention relates to the field of tissue sample preparation, in particular to a tissue transparent reagent and its preparation method and application. Background technique [0002] In medical and biological research, the transparency of biological tissue samples is helpful for doctors and scientific researchers to observe and study. In the field of transparent tissue sample preparation, the most widely used transparent agent is xylene. Xylene is a colorless, transparent, volatile liquid with an aromatic smell. It acts quickly and can be mixed with alcohol to dissolve paraffin. The gum used for sealing is mixed, but the water must be removed before use, otherwise milky turbidity will occur. In order to avoid material shrinkage, a gradual transition from pure alcohol to xylene should be adopted, that is, absolute alcohol → 1 / 2 absolute alcohol + 1 / 2 xylene → xylene. However, xylene is highly toxic and harmful to human health. It can enter the human...

Claims

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Application Information

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IPC IPC(8): G01N1/30
Inventor 王虎
Owner 王虎
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