High-efficiency and high-sensitivity biomacromolecule film transferring and dyeing system and equipment

Abstract

The invention discloses a high-efficiency and high-sensitivity biomacromolecule film transferring and dyeing system comprising a working unit provided with at least one passage used for detecting and analyzing a sample; and an operating part correlated with the working unit for creating at least one dyeing or film transferring passage parameter, wherein each dyeing or film transferring passage parameter comprises at least one electric field circulation time; and the invention further discloses equipment based on the high-efficiency and high-sensitivity biomacromolecule film transferring and dyeing system, and the equipment comprises a base and a shell, an operating panel is arranged at the outside of the shell, a working region, and a system power supply and a working circuit board, which are connected with the working region, are arranged in the base and the shell, the working region is provided with at least one push-pull type independent working chamber, and positive and negative electrode plates used for providing an electric field are installed in the working chamber. The quick dyeing or film transferring system or equipment capable of driving the movement of biological charged particles through the electric field to control the biomacromolecule disclosed by the invention can also guarantee the high sensitivity demand of biological detection.

Description

technical field [0001] The invention relates to the technical field of biological detection equipment, in particular to a biomacromolecule transmembrane transfer and dyeing system and equipment that utilize electric fields to drive the movement of biologically charged particles to achieve high efficiency and high sensitivity. Background technique [0002] In a DC electric field, the phenomenon that charged particles move to electrodes with opposite signs is called electrophoresis (electrophoresis). In 1809, the Russian physicist Peнce first discovered the electrophoresis phenomenon, but it was not until Tiselius in Sweden established the boundary electrophoresis (boundary electrophoresis) for separating proteins in 1937 that the electrophoresis technology began to be applied. In the 1960s and 1970s, when media such as filter paper and polyacrylamide gel were successively introduced into electrophoresis, electrophoresis technology developed rapidly. The rich and colorful ele...

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Problems solved by technology

Since the factors affecting the electrophoretic mobility are mainly related to the electric field strength, the pH value of the solution, and the ionic strength of the solution, the mobility of biomacromolecules and dyes in the medium where the electric field is applied is not the same, and biomacromolecules are larger. It moves slowly in the electric field and will not move out of the medium within a limited time. Organic dye molecules are much smaller than biomacromolecules, and dye molecules that are not combined with biomacromolecules can quickly move out of the medium, so they can be In the same electric field, the color development of biomacromolecules can be achieved by moving the dye out of the medium while the biomacromolecule (incorporated with the dye) remains in the medium, so that high resolution, little or no background influence can be obtained in a short time Analytical images of biomacromolecules in the background, but if the dye and biomacromolecules only meet and combine once, it is difficult to meet the requirements for some experiments with relatively high sensitivity requirements, and even prone to missed detection in the detection

[0004] With the rapid development of biomedicine and other disciplines, the detection technology of biomacromolecules is also rapidly improving. However, there is no system or device in the market that can use electric fields to drive the movement of biologically charged particles to control the rapid dyeing and transfection of biomacromolecules. membrane, and at the same time achieve the requirement of high sensitivity

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