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Insect in vitro labeling solution and its preparation method

A preparation method and external labeling technology, applied in the field of labeling liquid, can solve the problems of insect body wall injury, inability to accurately label small lepidopteran adults, insect damage and the like

Inactive Publication Date: 2013-02-20
孝感市野生动物和森林植物保护站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the paint markers are heavy and contain organic solvents, the solvent volatilization process will cause damage to the body wall of insects and affect the flight movement of insects, resulting in missing or distorted test data
Marker smearing can damage insects and prevent reliable and accurate marking of small adult Lepidoptera

Method used

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  • Insect in vitro labeling solution and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Dissolve 0.3 parts of biological staining agent (any one of acid fuchsin, fast green CFC, and crystal violet) in 80 parts of distilled water, shake and dissolve, and then dissolve 0.8 parts of Tween-20 in biological staining agent and In the mixed solution of distilled water, after shaking and dissolving, it is the insect in vitro labeling solution.

[0008] For example, dissolve 0.3 grams of acid fuchsin in 80 grams of distilled water in a biological stain, and shake to dissolve, then dissolve 0.8 grams of Tween-20 in a mixture of acid fuchsin and distilled water, shake and dissolve , which is the red insect in vitro marker solution.

Embodiment 2

[0010] Dissolve 0.5 parts of biological staining agent (any one of acid fuchsin, fast green CFC, and crystal violet) in 100 parts of distilled water, shake and dissolve, and then dissolve 1 part of Tween-40 in biological staining agent and In the mixed solution of distilled water, after shaking and dissolving, it is the insect in vitro labeling solution.

[0011] For example, dissolve 0.5 g of fast green CFC in 100 g of distilled water in biological staining agent, shake and dissolve, then dissolve 1 g of Tween-40 in the mixture of acid fuchsin and distilled water, shake and dissolve , which is the green insect in vitro labeling solution.

Embodiment 3

[0013] Dissolve 0.8 parts of biological staining agent (any one of acid fuchsin, fast green CFC, and crystal violet) in 120 parts of distilled water, shake and dissolve, and then dissolve 1.2 parts of Tween-60 in biological staining agent and In the mixed solution of distilled water, after shaking and dissolving, it is the insect in vitro labeling solution.

[0014] For example, dissolve 0.8 grams of crystal violet in 120 grams of distilled water in a biological stain, and dissolve by shaking, then dissolve 1.2 grams of Tween-60 in a mixture of acid fuchsin and distilled water, and shake and dissolve. It is the purple insect in vitro marker solution.

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Abstract

The invention relates to a labeling solution attached in vitro of insects the and its preparation method during studying the population quantity dynamics, behavior characteristics, activity rule and other related biological characteristics of insects. The invention is characterized in that the insect in vitro labeling solution is prepared from the following raw materials by weight ratio: 0.2-1.0 part of a biological coloring agent, 0.5-1.5 parts of Tween, and 80-120 parts of distilled water. The labeling solution has the beneficial effect that: it causes no chemical toxicity, physical injury and physiological interference to target insects, has no influence on the flight activity, feeding, mating oviposition and other normal biological habits of the target insects, the labeling solution can be firmly attached to a target labeling body and cannot be washed away by rain in the wild or disappear under sunshine and other natural conditions, i.e. the labeled object can maintain stable during studying and the use performance is reliable. The labeling solution has conveniently available raw materials, and the preparation method is simple, safety, and convenient to use and operate. When the target labeling object is recaptured, the inspection and identification method to a sample label is accurate and reliable, and the cost is low.

Description

technical field [0001] The invention relates to a marking liquid attached to insects outside the body and a preparation method thereof when studying insect population dynamics, behavior characteristics, activity rules and other related biological characteristics. Background technique [0002] Mark-recapture technology is one of the applied technologies in basic research of insect biology, ecology and behavior. It refers to collecting a certain number of target insects for individual marking in a specific area for a certain research need, and then putting them back into the natural population after mixing, recapturing a certain number of individuals, and then using them to recapture them according to statistical principles. To analyze the population dynamics, behavior characteristics, activity rules and other related biological characteristics of the research objects. At present, the most advanced insect in vivo labeling technology uses genetic methods such as RAPD markers, ...

Claims

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Application Information

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IPC IPC(8): C09B67/00A01K67/033
Inventor 丁强
Owner 孝感市野生动物和森林植物保护站
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