Method of analyzing target nucleic acid of biological samples

A nucleic acid sequence and target nucleic acid technology, applied in the field of target nucleic acid sequence analysis of biological samples, can solve problems such as laborious and time-consuming

Inactive Publication Date: 2013-02-27
蒂莫西刘
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Current methods of extracting and purifying nucleic acids from biological samples require laborious and time-consuming procedures that typically involve lysis of cells, precipitation of cell debris, followed by precipitation of DNA using chemical solvents and centrifugation or vacuum in multiple steps and in test tubes. resuspension

Method used

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  • Method of analyzing target nucleic acid of biological samples
  • Method of analyzing target nucleic acid of biological samples
  • Method of analyzing target nucleic acid of biological samples

Examples

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Embodiment 1

[0103] Example 1. SNP Analysis of Personalized Medicine

[0104] Warfarin is the most commonly prescribed anticoagulant for the treatment and prevention of arterial and venous thromboembolism. Warfarin has a narrow therapeutic dose range for different patients because various SNPs, including VKORC1, are known to affect warfarin sensitivity and can result in 35% to 50% variation in warfarin dose requirements sex. Warfarin sensitivity is detailed in US 2011 / 0236885A1 "Genetic Variants Predicting Warfarin Sensitivity" by Chen et al., the US patent application cited here being incorporated herein by reference. references. Furthermore, the FDA approved an updated label for warfarin in 2007, a move that highlighted an opportunity for health care providers to use genetic testing to improve initial drug dosage prediction for individual patients. (FDA, http: / / www.fda.gov / NewsEvents / Newsroom / PressAnnouncements / 2007 / ucm108967.htm).

[0105]In this example, the gene locus of VKORC1 / rs...

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Abstract

This invention provides a method of analyzing target nucleic acids of biological samples for multiplex nucleic acid analysis of disease associated genetic changes of biological samples in biomedical research and clinical diagnostics.

Description

[0001] This application claims the rights and priority of US Provisional Patent Application No. 61 / 473,182 filed on April 8, 2011, the entire content of which is incorporated herein by reference. technical field [0002] The present invention relates to the detection and analysis of nucleic acid molecules from biological samples, and in particular to the determination of single nucleotide polymorphisms (single nucleotide polymorphisms; SNP), mutations and other disease-related changes of target nucleic acid molecules. Background technique [0003] Genomic DNA can be used in various clinical diagnostic applications, such as DNA sequencing of relevant diseases, single nucleotide polymorphism (Single nucleotide polymorphism; SNP) genotyping and mutation screening. Information from such assays can be used for disease predisposition screening, disease treatment stratification, and personalized medicine management. After the completion of the human genome sequencing project ten ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6816C12Q2563/185C40B20/04C12Q1/68C12Q2565/518
Inventor 蒂莫西·Z·刘
Owner 蒂莫西刘
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