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Application of high-pass DNA (Deoxyribonucleic Acid) sequencing method on determination of short tandem repeat gene locus in human genome and method

A technology of DNA sequencing and tandem repetition, applied in the biological field, to achieve the effect of avoiding the connection step

Active Publication Date: 2013-02-27
IPE BIOTECHNOLOGY CO LTD
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Problems solved by technology

However, it is difficult to use fluorescence-labeled dideoxy-terminated capillary electrophoresis (Sanger method) for STR typing due to reasons such as throughput and cost, and it is difficult to become a practical method that can be used for daily determination, especially for database construction.

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  • Application of high-pass DNA (Deoxyribonucleic Acid) sequencing method on determination of short tandem repeat gene locus in human genome and method
  • Application of high-pass DNA (Deoxyribonucleic Acid) sequencing method on determination of short tandem repeat gene locus in human genome and method
  • Application of high-pass DNA (Deoxyribonucleic Acid) sequencing method on determination of short tandem repeat gene locus in human genome and method

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Embodiment Construction

[0077] The invention introduces the high-throughput DNA sequencing method into the STR determination technology, and can be used in forensic medicine and other related fields involving the detection of human STR loci, including genetic diagnosis, human population genetic research, gene mapping and other fields. In order to make the purpose, technical solution and advantages of the present invention clearer, the application in the field of forensic human STR site detection is taken as an example below, and the specific implementation of the present invention is described in conjunction with the accompanying drawings, and the content of the detection report compared with existing products More detailed advantages are described further.

[0078] 1. Materials

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Abstract

The invention discloses application of a high-pass DNA (Deoxyribonucleic Acid) sequencing method on determination of a short tandem repeat gene locus in human genome and a method, belonging to the technical field of biology. The method comprises the following steps of: preparing a multi-sample gene locus proliferation sublibrary; sequencing high-pass DNA; and analyzing data and reporting the result. According to the application and the method disclosed by the invention, the high-pass DNA sequencing technology is applied to determination of the human short tandem repeat (STR) gene locus for the first time and the resolving power and the sensitivity of the STR locus as individual human recognition are remarkably improved; and meanwhile, the detection pass of the STR is greatly improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the use and method of a high-throughput DNA sequencing method for determining short-segment tandem repeat loci in the human genome. Background technique [0002] How to track and identify suspects in forensic medicine, criminal investigation, and physical evidence identification; how to identify relevant personnel or victims in crimes, disasters, etc.; And the goal of continuous exploration. Since genetics became a discipline due to the groundbreaking work of G.J. Mendel more than 100 years ago, every progress in genetics has made the above work have a more solid scientific foundation. [0003] In 1985, the British geneticist Jeffreys (A. Jeffreys) first proposed the so-called "DNA fingerprint", pointing out that certain regions in the human genome have repetitive sequences, and such repetitive sequences have individual differences (polymorphism) and can be hereditary. There are m...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 周骋姚志建潘雅姣陈琛
Owner IPE BIOTECHNOLOGY CO LTD
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