Method for extracting natural anti-oxidizing substance from miracle fruit
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A natural anti-oxidation and miracle fruit technology, applied in food science, application, food preparation, etc., can solve the problems of extraction of biologically active substances and functional studies that have not been reported
Active Publication Date: 2013-03-13
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology
However, although many studies have been done on the mechanism of the fruit's flavor change, the extraction and functional studies of its bioactive substances have not been reported.
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Embodiment 1
[0025] (1) Put 2g of clean and pulverized miracle fruit pulp and 12ml of chromatographic grade ethanol in a glass test tube with a cover, mix evenly with a vortex instrument, and then carry out ultrasonic water bath leaching. Control the temperature of the ultrasonic water bath to 65°C and the ultrasonic time to 30min to obtain ethanol extract;
[0026] (2) After cooling the ethanol extract, centrifuge through a centrifuge, set the centrifuge speed to 5000rpm, centrifuge for 30min, and separate the supernatant;
[0027] (3) Repeat the above extraction and centrifugation steps once, and combine the supernatant;
[0028] (4) Add the above-mentioned combined supernatant to the treated C18 octadecyl solid-phase extraction column at a flow rate of 5ml / min, then wash the column with pure water, and then use 70v / v% ethanol aqueous solution with 5 Elution was performed at a flow rate of ±0.5ml / min, and the eluents were combined. All operations were carried out at 4°C and protected f...
Embodiment 2
[0032] (1) Put 2 g of clean and pulverized miracle fruit pulp and 18 ml of chromatographic grade ethanol into a glass test tube with a cover, mix them evenly with a vortex instrument, and then carry out ultrasonic water bath extraction. The temperature of the ultrasonic water bath is controlled at 45° C. Obtain ethanol extract;
[0033] (2) After the extract is cooled, it is centrifuged through a centrifuge, and the centrifuge speed is set to 5000rpm, centrifuged for 30min, and the supernatant is separated;
[0034] (3) Repeat the above extraction and centrifugation steps once, and combine the supernatant;
[0035] (4) Add the above combined supernatant to the treated C18 octadecyl solid phase extraction column at a flow rate of 5ml / min, then wash the column with pure water, and then elute with 80v / v% ethanol aqueous solution , combined eluents. All operations were carried out at 2°C and protected from light;
[0036] (5) Under normal pressure (1.01×10 5 Pa) evaporates eth...
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Abstract
The invention discloses a method for extracting a natural anti-oxidizing substance from a miracle fruit, comprising the following steps of: taking crushed miracle fruit pulp as a raw material and taking chromatograph-grade ethanol as a solvent; utilizing an ultrasonic water bath to immerse and extract to obtain an ethanol extraction solution; centrifugally separating the ethanol extraction solution to obtain liquid supernatant; repeatedly the steps of immersing and extracting, and centrifugally separating for at least one time and combining the liquid supernatants; adsorbing the combined liquid supernatant by using a non-polar silica gel substrate adsorbent and separating; utilizing an ethanol water solution to carry out elution and collecting an elution solution; distilling the elution solution to obtain a natural anti-oxidizing substance solution; and drying the natural anti-oxidizing substance solution in vacuum to obtain the solid natural anti-oxidizing substance. The extraction method has a simple step; and the extracted natural anti-oxidizing substance has a very strong anti-oxidizing biological activity function and has a wide application prospect.
Description
technical field [0001] The invention belongs to the field of extraction of natural substances, in particular to a method for extracting natural antioxidant substances from miracle fruit. Background technique [0002] Miracle fruit belongs to the mysterious genus of Sapotaceae, an evergreen shrub native to West Africa, distributed from West Africa to the Congo, and also found in the jungles of Indonesia. After being introduced in the 1960s, it was planted in Hainan, Guangdong, Guangxi, and Fujian in my country. Miracle fruit is a national treasure plant. No matter it is in West Africa or my country, it is protected and prohibited from exporting. [0003] The miracle fruit tree grows in the tropical area of West Africa. Its fruit is oval, with bright red skin when ripe, thin, milky white flesh, and slightly sweet taste. In addition, the miracle fruit pulp contains mysterious element, which can change the taste. Local residents often use it to adjust the taste of food. It ca...
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