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Method for rapidly exercising field melasma herb tissue culture seedlings

A technology of tissue culture seedlings and blood gallbladder, which is applied in the field of artificial planting of wild medicinal plants, can solve the problems of rapid propagation of blood gallbladder seedlings that have not been seen in public, and achieve low cost, simple technical methods, and convenient The effect of the operation

Inactive Publication Date: 2013-03-20
KUNMING UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] After searching the literature, I have not seen any published content about the rapid propagation of blood gallbladder seedlings, investigation of wild resources, seed germination, and wild transformation of domestic species.

Method used

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  • Method for rapidly exercising field melasma herb tissue culture seedlings
  • Method for rapidly exercising field melasma herb tissue culture seedlings
  • Method for rapidly exercising field melasma herb tissue culture seedlings

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Embodiment Construction

[0015] In order to make the purpose of the present invention and the technical solution clearer, the present invention uses specific examples to screen the seedlings for domestication, the management method of the tissue cultured seedlings before opening the bottle, the disinfection of the seedlings after opening the bottle, the regulation of light, temperature and water after planting, etc. The research screening process is fully described to justify the results of this research, but it is not intended that the invention be limited to these examples.

[0016] A method for rapid seedling hardening of tissue cultured seedlings of Melasma arvense (Benth.) Hand.-Mazz. ] comprising the following steps:

[0017] A. Substrate selection: After mixing peat, plant ash and fine sand 3:1:1 evenly, and passing through a 120-mesh sieve, the colonization substrate is prepared.

[0018] B. Substrate disinfection treatment: Spread the prepared substrate, spray 2000 times of carbendazim soluti...

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Abstract

The invention relates to a method for rapidly exercising field melasma herb tissue culture seedlings and belongs to the technical field of field melasma herb artificial cultivation. The method comprises six technical links of substrate formula selection, substrate sterilization, tissue culture seedling exercising management before seedlings come out of a bottle, bottle opening and seedling cleaning and sterilization, seedling exercising and planting method and management in a seedling exercising stage after planting; the field melasma herb tissue culture seedlings can be exercised and domesticated for 15-20 d; after the exercised and domesticated tissue culture seedlings are transplanted to big fields, the survival rate of the tissue culture seedlings is more than 95 %; the method provided by the invention has the advantages of high seedling exercising and domesticating speed, high seedling percentage and so on; and problems of lower field melasma herb tissue culture seedling transplanting survival rate, slow growth and the like are solved. The method provided by the invention has the advantages of simplicity, lower cost and convenience in operation and is suitable for being applied to actual production.

Description

Technical field: [0001] The present invention relates to a method for rapid hardening of tissue cultured seedlings of blood gallbladder, in particular to a method for rapid hardening of tissue cultured seedlings of wild medicinal plant Melasma arvense (Benth.) Hand.-Mazz. . It belongs to the technical field of artificial planting of wild medicinal plants. Background technique: [0002] Huaxuedan is the root of Melasma arvense (Benth.) Hand.-Mazz., a plant of the family Scrophulariaceae. It is a perennial herb, mainly distributed in the southern part of Yunnan Province, and belongs to the Miao, Yi, Lahu and other ethnic groups in Yunnan Province. Rare folk medicinal materials, many hospitals in Yunnan are mainly used to treat leukemia, tumors, cardiovascular and cerebrovascular diseases, icteric hepatitis, hepatomegaly, bruises, bruises, dysmenorrhea and other diseases. Huaxuebili has the potential to be developed as a special drug for treating early leukemia, anti-tumor, bl...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G1/00A01H4/00
Inventor 李育川王定康宋知春王海燕靳松
Owner KUNMING UNIV
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