Method for producing carotinoid and single-cell protein via transforming cellulose pyrolytical liquid and levoglucosan through photosynthetic bacteria
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A single-cell protein and photosynthetic bacteria technology, applied in the field of high value-added products produced by increasing photosynthetic bacteria, can solve the problems of restricting popularization and application, and high cost of culture medium
Inactive Publication Date: 2014-05-28
NANJING NORMAL UNIVERSITY
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Problems solved by technology
Generally, the cost of synthetic medium for photosynthetic bacteria is relatively high, which restricts its large-scale application
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Embodiment 1
[0023] Prepare a medium with 0.2% (W / W), pH 5.0, and inner ether sugar as the substrate. The specific formula is 0.2% inner ether sugar, 1% ammonium sulfate, and 0.1% KH 2 PO 4 , 0.5%MgSO 4 , 0.01%FeSO 4 . Insert purple photosynthetic bacteria (Thermochromatium tepidum), 30°C, 1500xl light, anaerobic static culture for 7 days, collect the bacteria by centrifugation at 5000rpm, break the wall with 3mol / L hydrochloric acid, extract with acetone to obtain carotenoids, and the assimilation rate of endosaccharides It was 79.4%, the amount of carotenoids was 401.3ug / g, and the single-cell protein yield was 1.61g / L.
Embodiment 2
[0025] Prepare 20% (W / W), pH 5.0, inner ether sugar as the substrate medium, the specific formula is 20% inner ether sugar, 1% ammonium sulfate, 0.1% KH 2 PO 4 , 0.5%MgSO 4 , 0.01%FeSO 4 Introduced purple photosynthetic bacteria (Thermochromatium tepidum), 30°C, 1500xl light, anaerobic static culture for 7 days, 5000rpm centrifugation to collect the bacteria, 3mol / L hydrochloric acid to break the wall, acetone extraction to obtain carotenoids, assimilation of endosaccharides The rate is 70%, the amount of carotenoids is 377.1ug / g, and the amount of single-cell protein is 1.34g / L.
Embodiment 3
[0027] Prepare a medium with 2% (W / W), pH 5.0, and inner ether sugar as the substrate. The specific formula is 2% inner ether sugar, 1% ammonium sulfate, 0.1% KH 2 PO 4 , 0.5%MgSO 4 , 0.01%FeSO 4. Introduced purple photosynthetic bacteria (Thermochromatium tepidum), 30°C, 1500xl light, anaerobic static culture for 7 days, 5000rpm centrifugation to collect the bacteria, 3mol / L hydrochloric acid to break the wall, acetone extraction to obtain carotenoids, assimilation of endosaccharides The rate was 79.2%, the amount of carotenoids was 517.6ug / g, and the amount of single-cell protein was 1.74g / L.
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Abstract
The invention relates to photosynthetic bacteria and applications thereof. Cellulose pyrolytical liquid and levoglucosan are utilized to culture the photosynthetic bacteria, and the obtained photosynthetic bacteria can be used for producing carotinoid and single-cell protein and also can be used as a water quality improving agent for aquaculture and sewage treatment or can be used as a leaf fertilizer and the like. The levoglucosan or the cellulose pyrolytical liquid is prepared to form a culture solution, under the conditions of certain loading volume and transformation temperature, the culture solution is cultured for certain time to obtain carotinoid-rich photosynthetic bacteria thallus, and the thallus which is subjected to carotinoid extraction can be used as a single-cell protein feed. The photosynthetic bacteria are utilized to effectively use the high-temperature pyrolysis products of cellulose biomass and the levoglucosan to prepare the carotinoid and the photosynthetic bacteria single-cell protein, the cellulose biomass is effectively utilized, the cellulose biomass with rich sources can be transformed into products with high additional values, wastes are turned into wealth, and the method has good economic and environmental application potentials and a social benefit.
Description
technical field [0001] The invention relates to a method for increasing photosynthetic bacteria to produce high value-added products by using the pyrolysis solution of cellulose biomass and inner ether sugar as raw materials, and specifically relates to the production of carotenoids by using photosynthetic bacteria to ferment cellulose pyrolysis solution and inner ether sugar and unicellular phage proteins. Background technique [0002] Carotenoids (carotenoids) are important natural pigments widely present in plants and microorganisms (Luo Xuan, Optimizing the Extraction Process of Rhodotorula carotenoids [J]. Biotechnology, 2007,17(4): 86-89). The reason why carotenoids have important economic value is that they not only have the function of preventing cancer and cardiovascular diseases, but also can be widely used in the fields of medicine, food and cosmetics (Wang Suilou. Liu Fengzhu. Gao Jianqi. Rhodotorula carotenoids Screening and application of carotenoid fermentat...
Claims
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