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Methods and compositions for protecting against neurotoxic agents

A technology of neurotoxicity and neurotoxin, which is applied in the field of anti-neurotoxic agents and compositions, and can solve problems such as failure to show beneficial effects

Inactive Publication Date: 2013-03-27
REVALESIO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dopamine agonists and monoamine oxidase type B inhibitors have shown that efficacy is inversely related to the incidence and severity of side effects, while studies of other treatment options, including coenzyme Q10, tocopherol (vitamin E), amantadine, and beta-blockers stanchants) trials either failed to show a beneficial effect or did not have sufficient data for a thorough assessment of risks and benefits

Method used

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  • Methods and compositions for protecting against neurotoxic agents
  • Methods and compositions for protecting against neurotoxic agents
  • Methods and compositions for protecting against neurotoxic agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0282] microbubble size

[0283] Experiments were carried out with gas-enriched liquids by using the diffuser of the present invention in order to determine gas bubble size limits. Microbubble size limits were established by passing gas-enriched liquids through 0.22 and 0.1 micron filters. In performing these tests, a volume of liquid is passed through the diffuser of the present invention and a gas-enriched liquid is produced. Draw sixty milliliters of this liquid into a 60-ml syringe. The dissolved oxygen level of the liquid in the syringe was then measured by Winkler titration. The liquid in the syringe was injected through a 0.22 micron Millipore Millex GP50 filter into a 50 ml beaker. The dissolved oxygen rate of the material in the 50ml beaker was then measured. The experiment was carried out three times and the results shown in Table 4 below were obtained.

[0284] Table 4

[0285] Dissolved Oxygen in Syringe

Dissolved oxygen after 0.22 micron filte...

Embodiment 2

[0289] (Patch clamp analysis of Calu-3 cells perfused with the electrokinetically generated fluids (RNS-60 and Solas) of the present invention showed that: (i) exposure to RNS-60 and Solas resulted in an increase in whole-cell conductance, (ii) exposure of cells to RNS-60 produced a non-linear increase in conductance, evident at 15 min incubation time, and (iii) exposure of cells to RNS-60 produced the effect of RNS-60 saline on calcium permeable channels)

[0290] overview. In this example, patch clamp studies were performed to further confirm the utility of the inventive electrokinetically generated saline fluids (RNS-60 and Solas) as described herein, including the utility of modulating whole-cell currents. Two sets of experiments were carried out.

[0291] Summary of data from the first set of experiments showed that whole cell conductance (current versus voltage) obtained with Solas saline was highly linear for both incubation times (15 min, 2 hr) and for all voltage reg...

Embodiment 3

[0317] (The electrokinetic fluids of the present invention were shown to be significantly effective in a dose-responsive manner in the art-recognized acute experimental allergic (autoimmune) encephalomyelitis (EAE) rat MBP model of multiple sclerosis (MS))

[0318] Overview:

[0319] In this working example, two regimens were administered prophylactically and therapeutically at two doses in the art-recognized rat model of myelin basic protein MBP-induced acute experimental allergic encephalomyelitis (EAE). The electrokinetic liquid RNS-60 of the present invention was evaluated in . The electrokinetic liquid RNS-60 of the present invention was shown to be significantly effective in a dose-responsive manner. Both therapeutic (daily administration of RNS-60 starting with MBP injection) and prophylactic (daily administration of RNS-60 starting seven days before MBP injection) RNS-60 dosage regimens showed significant reductions in clinical scores as well as delayed onset (in i...

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Abstract

Provided are methods for protecting against or reducing neurotoxicity of exposure to a neurotoxic agent, comprising administering an electrokinetically altered aqueous fluid as provided herein in an amount sufficient to provide for neuroprotection against the neurotoxic agent, preferably where protecting against or reducing loss of motor coordination in the subject exposed to the neurotoxin is afforded. In certain aspects, protecting or reducing neurotoxin-mediated neuronal apoptosis is afforded, and / or activating or inducing at least one of PI-3 kinase and Akt phosphorylation in neurons is afforded. Preferably, administering the fluid comprises administering the fluid prior to exposure to the neurotoxic agent. Additionally provided are methods for preserving or improving motor coordination in a subject having a neurodegenerative condition or disease, comprising administering an electrokinetically altered aqueous fluid as provided herein in an amount sufficient to provide for preserving or improving motor coordination in the subject.

Description

field of invention [0001] Certain aspects relate generally to methods of preventing or reducing neurotoxicity when exposed to a neurotoxic agent, comprising administering an electrokinetic altered aqueous fluid as provided herein, and preferably, wherein preventing in a subject exposed to a neurotoxin Or lower loss of motor coordination. Particular aspects relate to preventing or reducing neurotoxin-mediated neuronal apoptosis, and / or activating or inducing at least one of PI-3 kinase and Akt phosphorylation in neurons. Certain aspects relate generally to methods of preserving or improving motor coordination in a subject suffering from a neurodegenerative disorder or disease, comprising administering an electrokinetically altered aqueous fluid as provided herein. Cross references to related patent applications [0002] This patent application claims U.S. Patent Application No. 12 / 771,476, filed April 30, 2010 and entitled "COMPOSITIONS AND METHODS FOR TREATMENT OF NEURODE GE...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N39/00
CPCA61K9/0019A61K33/00A61K31/785A61K39/00A61K41/0004A61K9/0002A61K31/58A61K38/13A61K31/522A61K45/06A61K31/56A61P17/02A61P21/00A61P21/02A61P25/00A61P25/06A61P25/14A61P25/16A61P25/28A61P31/18A61P39/00A61P39/02A61P43/00A61P9/10A61K2300/00A61K9/16A61K9/14
Inventor 理查德·L·华森安东尼·B·伍德格雷戈里·J·阿咸宾
Owner REVALESIO CORP
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