Rapid propagation method for tissue cultivation of dendrobium candidum stem

Abstract

The invention relates to a rapid propagation method for tissue cultivation of a dendrobium candidum stem, belonging to the technical field of artificial cultivation of dendrobe. The method comprises the following steps of preparing an explant, inducing axillary bud and protocorm, conducting propagation cultivation, and rooting and seeding cultivation. The rapid propagation method has the beneficial effects that the technical problems of insufficient explant sterilization, high pollution rate, incapability of conducting axenic cultivation, slow protocorm differentiation, less differentiation, long propagation period, high production cost and the like can be well solved. Compared with the prior art, the rapid propagation method has the advantages that rapid propagation can be achieved; and the propagation period can be reduced by over 60 days. In addition, according to the rapid propagation method, seeding genetic traits are stable, the variation is small, the variety is pure, and the parent characteristics and features can be well kept. Thus, excellent seeding guarantee is provided for development of plant industry of dendrobium candidum, and the rapid propagation method has good economic benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of dendrobium candidum artificial cultivation, and in particular relates to a method for tissue culture and rapid propagation of stem segments of dendrobium candidum. Background technique [0002] In the production of Dendrobium officinale seedlings, the fruit (seed) is generally used as explants for rapid propagation. However, the seedlings bred by this method have limited traits of the mother body, large differences in traits, miscellaneous varieties, large variations, easy degradation, difficult quality assurance, and difficult maintenance of excellent seed properties. And when using stem tip or stem segment as propagation material, when carrying out tissue culture asexual rapid propagation, although can obtain the seedling with strong target selection, consistent character, consistent quality, high purity, but there is explant base number is small, difficult to sterilize , slow differentiation of protoc...

AI Technical Summary

Problems solved by technology

However, for the seedlings bred by this method, the traits of the mother body are limited, the traits are greatly different, the varieties are mixed, the variation is large, it is easy to degenerate, the quality is difficult to guarantee, and it is difficult to maintain good seed properties.

And when using stem tip or stem segment as propagation material, when carrying out tissue culture asexual rapid propagation, although can obtain the seedling with strong target selection, consistent character, consistent quality, high purity, but there is explant base number is small, is not easy to sterilize , slow differentiation of protocorms, long reproductive cycle, low multiplication factor, difficult establishment of sterile system and other technical problems, so it is not widely used in actual production