Rapid package removal method of digital microscopic holographic phase diagram

A digital microscope and phase map technology, applied in image analysis, image data processing, measuring devices, etc., can solve the problems of time-consuming sorting operations, time-consuming establishment of minimum span trees, and affecting the time to unpack

Inactive Publication Date: 2015-07-22
ZHEJIANG NORMAL UNIVERSITY
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Problems solved by technology

[0006] The currently widely used path tracing method is the flood algorithm of insertion sorting. This kind of sorting operation is very time-consuming, which affects the time to unpack
Another path tracing method is the path tracing method of the reliability tree, which omits the time of insertion sorting, but the operation of establishing the minimum span tree is still very time-consuming

Method used

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  • Rapid package removal method of digital microscopic holographic phase diagram
  • Rapid package removal method of digital microscopic holographic phase diagram
  • Rapid package removal method of digital microscopic holographic phase diagram

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Embodiment Construction

[0068] The present invention will be further described below in conjunction with the accompanying drawings.

[0069] We use figure 1 Experiments are carried out on the system shown. The wavelength of the light source used in the system is 0.532nm, the maximum power is 100mW, and the power is adjustable. The pixel size of the CCD camera used was 3.45 μm×3.45 μm, and the number of pixels was 512×512 pixels. The recording sample is red blood cells from a healthy human body. The numerical aperture of the selected microscope objective lens is 0.85, and the magnification is 60 times, which meets the requirements of the optimal design of the system's structural parameters. Two continuous attenuation sheets NF added in the optical path can further adjust the intensity of the object light wave and the reference light wave.

[0070] image 3 (a) is the acquired digital holographic micrograph of the image plane. Perform frequency domain filtering on the image plane hologram, and then...

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Abstract

The invention belongs to the field of digital holographic microscopic measurement and relates to a rapid package removal method of a digital microscopic holographic phase diagram. The method includes calculating and obtaining a reappearing intensity image and a package phase diagram according to a fundamental principle of digital holographic microscopy, calculating residual point distribution of the package phase diagram, automatically setting threshold value of intensity according to intensity value distribution of the pixels in the intensity image corresponding to the residual points, considering a pixel region which is higher than the threshold value as a reliable phase area, considering a pixel region which is lower than the threshold value as a suspicious phase area, placing a intensity value in a normalized intensity image corresponding to the reliable phase area as number 1, keeping the intensity value corresponding to the suspicious phase area to be unchanged, establishing a novel quality guiding image, and finally, establishing a stack chain table flooding algorithm based on guidance of the quality guiding image. Operating rate of the rapid package removal method of the digital microscopic holographic phase diagram is more than 100 times faster than that of a traditional plug-in sequencing flood algorithm with quality image guide, and is even about 3 times faster than that of a branch cutting algorithm. The rapid package removal method of the digital microscopic holographic phase diagram is a phase package removal method applied to quasi real-time digital holographic microscopy.

Description

technical field [0001] The invention belongs to the field of digital holographic microscopic measurement, and relates to a method for quickly unwrapping a digital microscopic holographic phase diagram. Background technique [0002] Digital holographic microscopy is the product of the combination of digital holography and microscopy technology. It can accurately obtain the phase distribution of object light waves, so it can analyze the three-dimensional surface morphology of tiny objects or the phase information of phase-type objects. It has been developed in recent years. It is an important microscopic imaging technology with broad application prospects and application fields. Phase unwrapping is an important issue in the research of digital holographic microscopy, because the phase information directly calculated by the complex amplitude is often wrapped in the main phase value of (-π, π], to obtain the real phase information, one must The phase is restored from the princi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01B11/24G06T7/00
Inventor 马利红李勇王辉金洪震
Owner ZHEJIANG NORMAL UNIVERSITY
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