Obesity gene susceptivity and pre-warning detection kit

A detection kit and obesity gene technology, applied in the field of genetics, can solve the problems of obesity, intracellular lipid accumulation, excessive proliferation of fat cells, etc.

Inactive Publication Date: 2013-05-01
SHANGHAI JINFANG BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As the increased expression of Gict2 molecule leads to hyperplasia of adipoc

Method used

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  • Obesity gene susceptivity and pre-warning detection kit
  • Obesity gene susceptivity and pre-warning detection kit
  • Obesity gene susceptivity and pre-warning detection kit

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with specific examples, and the examples are only for the purpose of illustration, and are not intended to limit the scope of the present invention. The experimental conditions and experimental methods that are not described in detail and specifically described in the following examples should be conventional experimental conditions and methods, or be carried out according to the operating steps and methods provided by the manufacturer.

[0036] Embodiment The use of this kit

[0037] 1. Obtaining the genomic DNA of the sample: rinse the mouth with water once or twice to remove food residues in the mouth, and pay attention to swallowing the remaining water in the mouth. Use a cotton swab to scrape off the exfoliated cells of the oral mucosa and put them into the cell fixative in an Eppendorf tube, and tighten the bottle cap for later use.

[0038] 2. DNA extraction and purification: use the cell lysis...

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Abstract

The present invention provides an obesity gene susceptivity and pre-warning detection kit, which is characterized in that a plurality of nucleic acid (DNA and RNA) molecule markers are combined, wherein the nucleic acid (DNA and RNA) molecule markers comprise APMI (+45), FTO (rs9939609), FTO (rs8050136), GNB3 (825C/T), LPL (HindIII), MC4R (rs17782313), PPARgamma2 (Pro12Ala), UCP2 (Ala55Val), and beta3-AR (Trp/Arg). The kit comprises: specific amplification primer pairs and DNA sequencing primer pairs of the gene polymorphism loci, PCR reaction regent components, PCR product enzymolysis regent components and DNA sequencing reaction regent components. According to the present invention, detection is performed by using the kit, and data processing and statistical analysis are performed on the related gene information, such that obesity susceptivity of a subject can be evaluated.

Description

technical field [0001] The present invention relates to the technical fields of genetics, molecular biology, clinical medicine and preventive medicine, specifically, the present invention relates to a kind of obesity gene susceptibility and early warning detection kit, through joint detection of multiple nucleic acid (DNA and RNA) molecular markers , including: APMI(+45), FTO(rs9939609), FTO(rs8050136), GNB3(825C / T), LPL(HindIII), MC4R(rs17782313), PPARγ2(Pro12Ala), UCP2(Ala55Val), β3-AR( Trp64Arg) gene polymorphism site, to assess and screen obesity genetic susceptibility (susceptibility). Background technique [0002] Obesity has become an increasingly serious "epidemic" in the world and has become an important global health problem. WHO pointed out that obesity is a disease, not only a matter of body shape and appearance, but also an independent risk factor for diabetes, cardiovascular diseases and even tumors. Obesity is often accompanied by insulin resistance, and oft...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 肖自力崔春黎纪燕燕
Owner SHANGHAI JINFANG BIOLOGICAL TECH
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