Preparation method of low molecular weight sea cucumber fucoidan sulfate
A fucoidan sulfate and low-molecular-weight technology is applied in the field of preparation of low-molecular-weight sea cucumber fucoidan sulfate. Free radical scavenging ability, good anticoagulant activity, little effect of product structure damage
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Embodiment 1-1
[0027] Embodiment 1-1, a preparation method of low-molecular-weight sea cucumber fucoidan sulfate, using sea cucumber polysaccharides obtained by enzymatic hydrolysis after pulverizing sea cucumbers as raw materials, the following steps are carried out in sequence:
[0028] 1) Dissolving sea cucumber polysaccharides in 3mol / L sodium chloride solution (concentrated brine) to obtain a sea cucumber polysaccharide solution, the mass concentration of sea cucumber polysaccharides in the sea cucumber polysaccharide solution is 1%;
[0029] Add ethanol to the sea cucumber polysaccharide solution until the final volume concentration of ethanol is 37%, centrifuge (6000r / min, 15min) to remove the precipitate, and obtain the supernatant;
[0030] Add ethanol to the supernatant until the final volume concentration of ethanol is 75%, centrifuge (6000r / min, 15min), and the resulting precipitate is dialyzed (through a dialysis bag with a molecular weight cut-off of 8000kDa) and freeze-dried (i...
Embodiment 1-2~1-5
[0035] In order to verify the effect of sonication with different ultrasonic field intensities on the prepared low-molecular-weight sea cucumber fucoidan sulfate in step 2), the ultrasonic field strength in step 2) was changed, and the rest were the same as in Example 1-1, so as to obtain Examples 1-2 to 1-5; the specific ultrasonic sound field intensity and the corresponding molecular weight of the obtained low molecular weight sea cucumber fucoidan sulfate are shown in Table 1.
[0036] Table 1. Molecular weight of low molecular weight sea cucumber fucoidan sulfate obtained by different ultrasonic sound field intensities
[0037]
[0038] It can be found from Table 1 that the ultrasonic sound field strength is 181.53-544.59W / cm 2 After 10 minutes of action, with the increase of ultrasonic sound field intensity, the molecular weight of sea cucumber fucoidan sulfate decreased continuously, concentrating on 138.47-217.48kDa. When the ultrasonic sound field intensity was 544.5...
Embodiment 2-1~2-3
[0040] In order to verify the effect of sonication at different reaction temperatures in step 2) on the prepared low-molecular-weight sea cucumber fucoidan sulfate, the reaction temperature in step 2) of Examples 1-4 was changed, and the rest were the same as in Example 1 -4, thereby obtaining embodiment 2-1 ~ 2-3 (that is, the treatment time is 10min, and the ultrasonic sound field intensity is 423.57W / cm 2 ); the specific reaction temperature and the corresponding molecular weight of the obtained low molecular weight sea cucumber fucoidan sulfate are shown in Table 2.
[0041] Table 2. Molecular weight of low molecular weight sea cucumber fucoidan sulfate obtained at different reaction temperatures
[0042]
[0043] It can be found from Table 2 that when the reaction temperature is 5-45°C, after ultrasonic treatment for 10 minutes, overall, the degradation trend increases as the temperature decreases, and the molecular weight of sea cucumber fucoidan sulfate is con...
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