Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for measuring resveratrol content in health-care products, food or raw materials

A technique for resveratrol and determination method, which is applied in the directions of measurement device, material separation, analysis material, etc., can solve the problems of long time consumption, cumbersome determination steps, distorted determination results, etc., and achieves high sensitivity, good selectivity and specificity. , the effect of eliminating false positives

Inactive Publication Date: 2013-05-15
TIANJIN TIANSHI BIOLOGICAL DEV +2
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to solve the problems in the prior art that the determination steps are cumbersome and time-consuming, and the isomerization of resveratrol under ultraviolet irradiation will cause distortion of the determination results, and provide a health product, food or raw material. Determination method of resveratrol content

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring resveratrol content in health-care products, food or raw materials
  • Method for measuring resveratrol content in health-care products, food or raw materials
  • Method for measuring resveratrol content in health-care products, food or raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Take grape seed extract as the sample to be tested, weigh 0.5g of the sample to be tested into a 100mL volumetric flask, add 40mL of methanol to dissolve, treat it in an ultrasonic water bath for 20min, let it stand and cool to room temperature, and use a centrifuge at 5000r / min Centrifuge for 5 minutes, filter with fast qualitative filter paper, dilute the filtrate to the mark with methanol, and shake well;

[0039] (2) Get the solution obtained in step (1) and dilute 5000 times with methanol to become the sample solution to be tested;

[0040] (3) The sample liquid to be tested is injected into a high-performance liquid chromatography-mass spectrometer, and the reverse phase C18 chromatographic column is used for chromatographic separation, and the mass spectrometer detects;

[0041] (4) Accurately weigh 10 mg of the resveratrol standard substance in a 100 mL volumetric flask, and use chromatographic methanol to ultrasonically dissolve and set the volume to the sc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for measuring resveratrol content in health-care products, food or raw materials. The method comprises the following steps of: weighing a sample to be measured, adding methanol in the sample to be measured, treating in an ultrasonic water bath, standing, cooling to the room temperature, centrifuging, filtering, and conducting constant volume on the filtrate by using methanol; diluting the filtrate after the constant volume into a sample solution to be measured by using the methanol; filling sample solution to be measured into a high-performance liquid chromatography-mass spectrometer to be detected; precisely weighing a resveratrol standard substance, putting the resveratrol standard substance into a volume bottle, dissolving the resveratrol standard substance and conducting the constant volume on the dissolved resveratrol standard substance to a scale in a chromatograph methanol ultrasonic mode, so as to be used as a standard stock solution; respectively diluting by using the methanol, and preparing into solutions respectively with the concentration being 0.1 microgram / mL, 0.2 microgram / mL, 0.4 microgram / mL, 0.8 microgram / mL and 1 microgram / mL; respectively filling the solutions into the high-performance liquid chromatography-mass spectrometer to be detected, so as to obtain standard curves; and calculating acquired inspection data according to the standard curves so as to obtain the concentration of resveratrol in the sample to be measured. The method disclosed by the invention is good in accuracy, high in precision and good in linearity, and is convenient to conduct measurement on the resveratrol content in health-care products, food or raw materials.

Description

technical field [0001] The invention relates to a method for detecting the content of resveratrol, in particular to a method for detecting the content of resveratrol in health products, foods or raw materials by using a liquid chromatography-mass spectrometer. Background technique [0002] Resveratrol (English name is Resveratro1) chemical name: (E)-5-[2-(4-hydroxyphenyl)-vinyl]-1,3-benzenediol; 3,5,4''- Trihydroxystilbene; stilbene triphenol; Trans-3-4'-trihydroxystilbene; Molecular formula: C14H1203; Molecular weight: 228.25. [0003] Physical properties: odorless, white crystal powder; insoluble in water, easily soluble in ethanol, acetone and other organic solvents. [0004] Resveratrol is a natural antioxidant that can reduce blood viscosity, inhibit platelet aggregation and vasodilation, keep blood flowing, prevent the occurrence and development of cancer, and have anti-atherosclerosis, coronary heart disease, ischemia Sexual heart disease, prevention and treatment o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/88G01N30/06G01N30/89
Inventor 赵晓娟李星芝孙俊峰
Owner TIANJIN TIANSHI BIOLOGICAL DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com