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Medicine application of chloranthus japonicus alcohol M

A technology of silverwort and medicine, applied in the field of application of silverwort M in the preparation of anti-tumor drugs, achieving the effects of small side effects, inhibition of proliferation, and obvious tumor-suppressing effect

Inactive Publication Date: 2013-06-05
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Medicine application of chloranthus japonicus alcohol M

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 MTS method to determine the growth inhibitory effect of silver lintol M on liver cancer cells

[0034] HepG2 (purchased from ATCC) 3×10 3 Inoculate each well into a 96-well plate, culture for 24 hours to make it adhere to the wall, and add biltiol M (purchased from Shanghai Institute of Materia Medica, Chinese Academy of Sciences), set 6 concentration gradients, and set 3 multiple wells for each concentration. Cells at 37°C, 5% CO 2 After culturing for 72 hours under the conditions, the culture medium was discarded, and the cell survival rate was measured with MTS kit (Promega).

[0035] The test method is: wash the cells once with serum-free medium, add the pre-prepared MTS coloring solution (add 2ml solution 1 and 100 μl solution 2 to 10ml serum-free medium, mix well) according to the amount of 100μl / well. Set a hole without cells as the background hole to correct the background light absorption of the solution. Put the cells in the cell incubator and continue t...

Embodiment 2

[0039] Example 2 Inhibitory effect of silverine alcohol M on the growth of human pancreatic cancer cells

[0040] Use cck-8 kit (Japan Tongren Chemical Research Institute) to detect.

[0041] step:

[0042] 1) Plant PANC-1 cells (purchased from the Cell Bank of the Chinese Academy of Sciences) evenly in a 96-well plate, the number of cells per well is 3×10 3 One.

[0043] 2) After sticking to the wall, add medicine after overnight, and add medicine (the concentration of silvergrass alcohol M is 50, 16.67, 5.56, 1.85, 0.62 μM respectively), each concentration has 3 multiple holes.

[0044] 3) Cultivate for 48 hours, replace the complete medium with a mixture of serum-free medium and CCK8 (10:1), and incubate for 2 hours in an incubator at 37°C.

[0045] 4) Take 450nm as the measurement wavelength and 650nm as the control wavelength, and measure the reading on the microplate reader.

[0046] Result: The IC50 value of silver lintol M on PANC-1 cells was 11.05μM.

Embodiment 3

[0047] Example 3 The inhibitory effect of leucocephalus M on the growth of human leukemia cells

[0048] According to the method of Example 2, the effect of giltwick M on K562 cells was detected, and the results showed that the IC50 value of giltwick M on K562 cells was 15.83 μM.

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Abstract

The invention belongs to the fields of chemical industry and medicine, and relates to an application of chloranthus japonicus alcohol M in preparation of anti-neoplastic drugs. The invention provides the application of the chloranthus japonicus alcohol M in preparation of the anti-neoplastic drugs. Cancer cells comprise hepatoma carcinoma cells, cervical cancer cells, leucocythemia cells, and pancreatic cancer cells. The chloranthus japonicus alcohol M belongs to a natural product, is low in toxic side-effect, high in bioavailability, and stable in property, and has clinical use value. A small molecule compound of the chloranthus japonicus alcohol M is used as a novel anti-neoplastic drug or an auxiliary element of the novel anti-neoplastic drug to be developed, tumor suppression effect is obvious, green environmental protection is achieved, and a novel method and a novel means are supplied for tumour treatment and cure.

Description

Technical field [0001] The present invention belongs to the field of chemical industry and medicine, and relates to the application of giltgrass alcohol M in the preparation of anti-tumor drugs. Background technique [0002] Silver thread grass is the whole plant or roots and rhizomes of Chloranthus Japonicus Sieb (Chloranthus Japonicus Sieb). Sesquiterpenoids are a relatively important class of compounds in Chrysospermum orchidaceae. They are distributed in the entire Chrysanthemum orchidaceae plants and have biological activities such as anti-inflammatory, antispasmodic and inhibiting microorganisms. The semiterpenoids obtained from silvergrass have complex structures and their skeletons can be divided into several types such as eucalyptane type, cyclocine type, gemane type, and calamus type. At the same time, they have lactones, ketones, alcohols, and more. Aggregate and other forms. [0003] The structural formula of silvergrass alcohol M is as follows: [0004] [0005] In 20...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61P35/00A61P35/02
Inventor 余龙朱恒锐刘祖龙胡立宏
Owner FUDAN UNIV
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