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Analytic method of flue-cured tobacco volatile aroma components

An aroma component and analysis method technology, applied in the field of chemical analysis, can solve the problems of loss of volatile aroma components, limited chromatographic peak capacity, insufficient resolution, etc., to improve accuracy and reliability, improve resolution, and shorten analysis time Effect

Active Publication Date: 2013-06-05
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention: The present invention aims at the problems in the existing analysis technology of aroma components of tobacco leaves that the extraction method is easy to cause the loss or decomposition of some volatile aroma components, the limited capacity of chromatographic peaks and insufficient resolution in the separation and identification method, etc., the present invention provides a headspace solid The method of phase microextraction combined with comprehensive two-dimensional gas phase time-of-flight high-resolution mass spectrometry for the determination of volatile aroma components in tobacco leaves can be used for the analysis and evaluation of aroma components in first-cured tobacco leaves

Method used

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  • Analytic method of flue-cured tobacco volatile aroma components
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  • Analytic method of flue-cured tobacco volatile aroma components

Examples

Experimental program
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specific Embodiment approach

[0016] detailed description: The present invention is further described by the following specific examples, but does not limit the present invention.

[0017] Cut the flue-cured tobacco leaves into shredded tobacco and equilibrate for more than 48 hours at a temperature of 22°C ± 1°C and a relative humidity of 60% ± 2%. Weigh 20-50 mg of the above-mentioned cut tobacco into a 15-22 mL headspace bottle, add 2-10 ng of phenethyl acetate as an internal standard, and seal it with a headspace bottle cap. The above samples were subjected to headspace solid-phase micro-extraction with a manual solid-phase micro-extraction device. The conditions of the solid-phase micro-extraction device were: fiber head DVB / CAR / PDMS; equilibrium temperature: 60-80 ℃; equilibrium time: 20-40 min; The adsorption time is 5-10 min; the desorption temperature is 250-270℃; the desorption time is 2-5 min. Finally, the full two-dimensional gas phase time-of-flight high-resolution mass spectrometry was used...

Embodiment 1

[0019] Example 1: Determination of repeatability and accuracy:

[0020] Using the above-mentioned inventive method, the same sample was tested in parallel 5 times a day for 3 consecutive days, and the intra-day and inter-day relative standard deviations were calculated respectively, and the positive and negative matching degree and accurate molecular weight deviation were investigated. The results are shown in Table 1.

[0021]

[0022]

[0023] After a full two-dimensional gas phase time-of-flight high-resolution mass spectrometry separation and identification, a total of 74 volatile aroma components in the flue-cured tobacco were identified. Confirmed by high resolution, the exact molecular weight difference is less than 10 mmu, and the exact molecular weight deviation of most target compounds is less than 5 mmu. At the same time, combined with the positive similarity and negative similarity searched by the standard library NIST08, the positive similarity There are 26 compoun...

Embodiment 2

[0026] The method of the present invention is used to determine the volatile aroma components of flue-cured tobacco samples from different regions of China, including 5 samples from Guiding in Guizhou, Xianfeng in Hubei, Sanming in Fujian, Shizong in Yunnan, and Queshan in Henan, and 74 volatile tobacco leaves were detected by the above method The content of sexual aroma components is shown in Table 2:

[0027]

[0028]

[0029]

[0030] The above-mentioned samples are all the middle leaves of the fresh-cured tobacco leaves with a grade of C3F. The samples used are fresh-cured tobacco leaves before being processed. The content of volatile aroma components may change after the tobacco leaves are redryed or flavored.

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Abstract

The invention provides an analytic method of flue-cured tobacco volatile aroma components. A measurement method that a headspace solid phase micro-extraction technology and a full-two-dimensional gaseous phase flight time high-resolution mass spectrometer are combined and used for qualitative analysis of the flue-cured tobacco volatile aroma components and quantitative analysis of the flue-cured tobacco volatile aroma components, effectively avoid loss of the flue-cured tobacco volatile aroma components or degradation of the flue-cured tobacco volatile aroma components, and guarantee original aroma characteristics of tobacco. The method is simple and practical in design, capable of simultaneously analyzing dozens of volatile aroma components of flue-cured tobacco, good in repeatability, high in analysis sensitivity, simple to operate, short in analysis time and applied to analysis of large-batch samples.

Description

technical field [0001] The invention belongs to the field of chemical analysis, and relates to an analysis method for volatile aroma components of uncured tobacco leaves, in particular to a headspace solid-phase microextraction combined with comprehensive two-dimensional gas-phase time-of-flight mass spectrometry for analyzing volatile aroma components in uncured tobacco leaves. Volatile aroma components. Background technique [0002] The aroma of tobacco is the result of the joint action of a variety of aroma components with specific aroma characteristics. Different types of tobacco varieties or varieties of the same type have different composition, content and proportion of aroma components under different ecological environments and cultivation conditions. , thus representing different aroma types. At present, according to the aroma performance of different origins and varieties of flue-cured tobacco in my country, the aroma of flue-cured tobacco in my country is divided...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 向章敏蔡凯耿召良周淑平葛永辉
Owner GUIZHOU TOBACCO SCI RES INST
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