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Application of Anoplin in treatment of esophageal cancer

A melittin antibacterial peptide, esophageal cancer technology, applied in the field of relieving cancer symptoms in subjects or inhibiting cancer cell proliferation, and treating cancer in subjects

Active Publication Date: 2014-11-12
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no literature report whether the smallest linear polypeptide found in nature has anti-tumor effect

Method used

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  • Application of Anoplin in treatment of esophageal cancer
  • Application of Anoplin in treatment of esophageal cancer
  • Application of Anoplin in treatment of esophageal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] 1. Materials and methods

[0033] 1.1 Materials, instruments and reagents

[0034] 1.1.1 Cell lines and peptides

[0035] Human chronic myelogenous leukemia K562 cells, mouse erythroleukemia MEL cells, human esophageal cancer TE-1 cells and human liver cancer Bel-7404 cells were purchased from the Chinese Academy of Medical Sciences Tumor Cell Bank. The antibacterial peptide Anoplin was synthesized by Hangzhou Zhongpei Company by solid-phase peptide synthesis.

[0036] 1.1.2 Main reagents

[0037] DMEM medium Gibco company

[0038] FBS Hyclone

[0039] Trypan Blue Sigma

[0040] MTT Sigma

[0041] DMSO (dimethyl sulfoxide) Sigma-Aldrich

[0042] PI Lianke Biological Co., Ltd.

[0043] Hoechst33342 Biyuntian Biotechnology Co., Ltd.

[0044] Cell Cycle Detection Kit KGI Biotech Co., Ltd.

[0045] Apoptosis Detection Kit Bebo Biotech Co., Ltd.

[0046] 1.1.3 Reagent preparation

[0047] Phosphate buffer saline (PBS): 8g sodium chloride, 0.2g potassium chloride...

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Abstract

The invention discloses an application of Anoplin in treatment of esophageal cancer, in particular relates to a method for treating the esophageal cancer of a subject, relieving symptoms of the esophageal cancer of the subject or inhibiting the proliferation of esophageal cancer cells, and the application of Anoplin in preparing medicaments, agents or medicament compositions for treating the esophageal cancer, relieving the symptoms of the esophageal cancer or inhibiting the proliferation of esophageal cancer cells, and relates to a pharmaceutical product including Anoplin which is used as an active ingredient and a specification which relates to the pharmaceutical product.

Description

technical field [0001] The invention relates to the antitumor effect of the mee venom antibacterial peptide Anoplin. Specifically, the present invention relates to: methods for treating cancer in a subject, relieving cancer symptoms in a subject, or inhibiting cancer cell proliferation; Use in medicine, medicament or medicine combination; and relates to a pharmaceutical product, which includes the melittin antimicrobial peptide Anoplin as an active ingredient and includes instructions related to the pharmaceutical product. Background technique [0002] Cancer is a kind of disease that seriously endangers human health. At present, the global cancer death toll is higher than the sum of the death toll from AIDS, tuberculosis and malaria, and it has become the world's number one killer [1] . The number of people who die of malignant tumors in the world exceeds 7 million every year, and there are about 1.6 million people in China. According to John Severin, head of the America...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/08A61P35/00
Inventor 付彩云卢燕朱丽娜赵辅昆张世馥
Owner ZHEJIANG SCI-TECH UNIV