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Methods and related compositions for reduction of fat and skin tightening

a skin tightening and fat reduction technology, applied in the field of methods, can solve the problems of excessive fluid loss, shock or fluid accumulation that must be drained, friction burns, etc., and achieve the effect of tightening a loose skin region

Inactive Publication Date: 2006-06-15
RGT UNIV OF CALIFORNIA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0013] The compositions (e.g., solutions) include a therapeutically effective amount of the pharmacological detergents (e.g., bile acid(s) and / or bile salt(s). Such therapeutically effective amounts are effective to reduce a subcutaneous fat deposit or tighten a region of loose skin.
[0028] In some embodiments, the skin tightening solution includes a therapeutically effective amount of the pharmacological detergents (e.g., bile acid(s) and / or bile salt(s). Such therapeutically effective amount is effective to tighten a loose skin region.

Problems solved by technology

However, liposuction and other surgical methods of fat removal are associated with significant adverse events including temporary bruising, swelling, numbness, soreness and burning sensation, risk of infection, pigmentation changes, the formation of fat clots or blood clots which can migrate to the lungs and cause death, excessive fluid loss, which can lead to shock or fluid accumulation that must be drained, friction burns or other damage to the skin or nerves or perforation injury to the vital organs.
Additionally, liposuction requires a recovery time of one to two weeks wherein the patient cannot work or perform certain daily activities.
Moreover, because surgical procedures such as liposuction require local and occasionally general anesthesia, significant anesthesia-related risks are associated with surgical fat removal.
Furthermore, liposuction and other drastic weight loss methods may result in loose skin.

Method used

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  • Methods and related compositions for reduction of fat and skin tightening
  • Methods and related compositions for reduction of fat and skin tightening
  • Methods and related compositions for reduction of fat and skin tightening

Examples

Experimental program
Comparison scheme
Effect test

example 1

Sodium Deoxycholate and Phosphatidylcholine Formulations

[0183] Phosphatidylcholine bile salt formulation (PBF) (5.0% highly purified soy derived PC, 4.75% sodium deoxycholate, and 0.9% benzyl alcohol, in sterile water, Table 2) was obtained from Hopewell Pharmacy, Hopewell, N.J. Sodium deoxycholate and Triton X-100 detergent (Triton, alkylaryl polyether alcohol) were obtained from Sigma-Aldrich Corp. (St. Louis, Mo.). Empigen BB detergent (Empigen, lauryldimethylbetaine) was obtained from Calbiochem, Biosciences, Inc., (La Jolla, Calif.). An injectible PBF solution was made according to Table 2 below. Stock reagents (5% dilutions) were prepared in PBS buffer.

TABLE 2Injectable PBFPhosphatidylcholine5.00% (w / v)Sodium deoxycholate4.75%Benzyl alcohol0.90%Water100 mL

[0184] The molecular structure of (a) phosphatidylcholine, (b) sodium deoxycholate and (c) benzyl alcohol are depicted in FIG. 1.

example 2

Effects of Sodium Deoxycholate and Phosphatidylcholine Solutions in Cultured Cells

[0185] To measure cell viability after detergent treatment, HaCaT human keratinocyte cells were cultured in DMEM (Dulbecco's modified Eagle's medium) supplemented with 10% fetal calf serum, penicillin, and streptomycin. HaCaT cells were cultured in 6 well plates and incubated with 0, 0.005, 0.050 or 0.500% of the PBF or sodium deoxycholate for 30 min at 37° C. prior to determination of cell viability using the MTS assay, which uses a tetrazolium compound that produces a color change when bioreduced by metabolically active cells (CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay, Promega, Corp. Madison, Wis.). Cell viability was determined by an absorbance spectrophotometer (at 490 nm) after 4 hour incubation with the assay at 37° C. To determine cell viability in fresh tissue, fat specimens were incubated for 4 hours in 24 well plates with stock reagents and the MTS assay. Tissue specimens...

example 3

Effects of Sodium Deoxycholate and Phosphatidylcholine Solutions in Porcine Tissue

[0187] Porcine tissue was obtained immediately after sacrifice, shaved, and placed on ice for a maximum of four hours before use. Fat specimens were obtained by removing the epidermis and dermis of a punch biopsy with a scalpel and trimmed. Fat specimens were loaded with calcein dye by incubating 1 hour at 37° C. with Calcein-AM (Sigma). Stock reagents were added to the fat specimens and incubated for 30 min at 37° C. with gentle agitation. Calcein retention was determined by tissue fluorescence using purple (411 nm) light and visually observing the emitted green (500 nm) light using an emission filters.

[0188] Histology was performed by injecting stock reagent solutions (0.5 mL) into full thickness porcine skin at various levels (epidermis, dermis, and subcutaneous tissue) with 1.0 mL syringes and 30-gauge, 0.5 inch needles. Needle depth was visualized along the margin of the porcine tissue with the ...

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Abstract

Compositions and methods useful in the reduction of localized fat deposits and tightening of loose skin in subjects in need thereof using pharmacologically active detergents are disclosed. The pharmacologically active detergent compositions can additionally include anti-inflammatory agents, analgesics, dispersion or anti-dispersion agents and pharmaceutically acceptable excipients. The pharmacologically active detergent compositions are useful for treating localized accumulations of fat including, for example, lower eyelid fat herniation, lipodystrophy and fat deposits associated with cellulite and do not require surgical procedures such as liposuction.

Description

CROSS-REFERENCE [0001] This application is a continuation-in-part application of Ser. No. 11 / 134,727, filed May, 19, 2005 which is a continuation-in-part application of Ser. No. 11 / 054,171, filed Feb. 8, 2005, which claims priority to U.S. Provisional Application Ser. No. 60 / 572,879 filed May 19, 2004, which are incorporated herein by reference in its entirety.INCORPORATION OF REFERENCE [0002] All references disclosed herein are incorporated in their entirety by reference for any and all purposes. BACKGROUND OF THE INVENTION [0003] Surgical and non-surgical procedures for improving appearance have increased in prevalence as populations age and gain weight. Liposuction is a popular cosmetic surgery procedure and involves the surgical removal of fat deposits using suction and optionally assisted by solutions to assist in fat removal. Liposuction is a surgical procedure that removes fat through an incision in the skin through which a cannula is inserted. The cannula is connected to a s...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/685A61K31/56A61K9/127A61K31/185
CPCA61K8/63A61K35/35A61K31/185A61K31/56A61K31/685A61K47/24A61K47/28A61K2800/91A61Q19/06A61Q19/08A61K9/0019A61K31/575A61K45/06A61K2300/00A61P3/04
Inventor KOLODNEY, MICHAEL S.ROTUNDA, ADAM M.
Owner RGT UNIV OF CALIFORNIA
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