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Primary homologous three-cell four-dimensional model of pharmaceutical research on central nervous system and construction method

A central nervous system and four-dimensional model technology, applied in the field of the original homologous three-cell four-dimensional model and construction of central nervous system drug research, can solve problems such as ignoring system relationships, and achieve the effect of avoiding differences

Inactive Publication Date: 2013-07-03
DALIAN MEDICAL UNIVERSITY
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Problems solved by technology

This method only focuses on the interaction between astrocytes and capillary endothelial cells or neurons in the central nervous system, but also ignores the interaction among capillary endothelial cells, astrocytes and neurons system relationship

Method used

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  • Primary homologous three-cell four-dimensional model of pharmaceutical research on central nervous system and construction method
  • Primary homologous three-cell four-dimensional model of pharmaceutical research on central nervous system and construction method
  • Primary homologous three-cell four-dimensional model of pharmaceutical research on central nervous system and construction method

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Embodiment Construction

[0023] The construction method of the primary homologous three-cell four-dimensional model of the central nervous system drug research of the present invention is carried out as follows:

[0024] a. The astrocytes, capillary endothelial cells and neuron cells of the cerebral cortex of SD rats were extracted and primary cultured respectively;

[0025] The specific operation is as follows:

[0026] a1. Astrocytes from the cerebral cortex of SD rats (A) were extracted and cultured in primary culture

[0027] Extract the cerebral cortex of a newborn SD rat within 24 h according to the method of the prior art, rinse the cortical tissue with D-Hanks solution for 3 times, and cut the cerebral cortex to 1mm 3 For the left and right small pieces, add 500 μl of trypsin and 500 μl of D-Hanks with a mass concentration of 0.25% and incubate for 30 minutes in a 37°C incubator; after 30 minutes, carefully transfer the tissue piece to a container containing 2 ml In the test tube of astrocyt...

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Abstract

The invention discloses a primary homologous three-cell four-dimensional model of the pharmaceutical research on a central nervous system, and a construction method. The primary homologous three-cell four-dimensional model of the pharmaceutical research on the central nervous system is characterized in that neuron cell cultures and a neuron cell culture medium are contained in a 24-mesh culture plate; a Transwell Insert is inserted into the neuron cell culture medium; the inner chamber surface of a filter membrane of the Transwell Insert is coated with collagen IV; capillary endothelial cell cultures are contained in the inner chamber surface of the Transwell Insert; astroglia cell cultures are contained in the outer chamber surface of the Transwell Insert; protrusions of astroglia cell cultures penetrate into the capillary endothelial cell cultures through filter membrane pore size of the Transwell Insert; and the neuron cell culture medium comprises components in percentage by volume as follows: 98% of the cell culture medium, 1% of a double-antibody and 1% of L-glutamine.

Description

technical field [0001] The invention belongs to the technical field of drug research model construction for central nervous system protection and damage repair, in particular to a drug based on capillary endothelial cells (endothelial cells, E), astrocytes, A and neuron cells in the central nervous system (neuron, N) Primary homologous three-cell four-dimensional model and construction method for central nervous system drug research formed by three basic cells. Background technique [0002] The central nervous system is the most important and complex living tissue in the world, and central nervous system diseases are one of the health burdens and medical problems. Therefore, research on drugs for central nervous system protection and damage repair has always been a hot spot and difficulty in the field of biomedical research , the technical bottleneck is the urgent need to establish a cell growth carrier that can not only maintain the normal shape and function of cells in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/079C12N5/0793C12Q1/02
Inventor 孙长凯李彧媛郑铁铮范明赵慧
Owner DALIAN MEDICAL UNIVERSITY
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