Method for detecting quorum sensing quenching bacterial strain

Abstract

The invention relates to a method for detecting a quorum sensing quenching bacterial strain. The method comprises the steps of: adding a bacterial strain to be detected in a PIPES (1,4-piperazinediethanesulfonic acid) buffer solution with a pH value of 6.7 to prepare a solution to be detected, wherein AHL (N-acyl Homoserine Lactone) is dissolved in the PIPES buffer solution, then enabling supernatant of the solution to be detected to react with a bacterial solution of A.tumefaciens A136 added with X-gal; after the reaction is ended, detecting OD492 and OD630 (Optical Density) values of a reaction solution, and calculating a standardized beta-galactosidase activity value through a formula of (0.653*OD492-OD630) / (0.267*OD630-OD492), and carrying out different significance analysis through comparing the standardized beta-galactosidase activity value, and finally, determining whether the bacterial strain to be detected is a quorum sensing quenching bacterial strain. The method provided by the invention has the advantages of being simple and rapid, being high in sensitivity, achieving high throughput detection, eliminating most of false positive bacterial strains, and being capable of effectively determining whether the bacterial strain to be detected is the quorum sensing quenching bacterial strain, thereby remarkably improving the accuracy and the pertinence of screening the quorum sensing quenching bacterial strain and saving the time and labor cost.

Description

technical field [0001] The invention belongs to the technical field of aquatic microorganism detection, and in particular relates to a detection method for density induction quenching bacteria. Background technique [0002] With the rapid development of the domestic marine aquaculture industry, the scale of aquaculture is expanding and the degree of intensification is also increasing. However, a series of problems such as the pollution of aquaculture water and the proliferation of harmful bacteria continue to appear, resulting in huge losses for my country's mariculture industry. At present, the control of diseases ensures a healthy breeding environment by improving the quality of breeding water and reducing the breeding density on the one hand, and on the other hand improves the disease resistance of farmed animals by means of vaccination and the use of immune enhancers. But when the disease occurs on a large scale, the use of broad-spectrum antibiotics is still the most c...

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a detection method for density induction quenching bacteria, that is, a simple high-throughput detection method for density induction quenching bacteria, thereby overcoming the shortcomings of current methods such as tediousness, inaccuracy, and high false positive rate

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